Enhancing osteogenesis of adipose-derived mesenchymal stem cells using gold nanostructure/peptide-nanopatterned graphene oxide.

Colloids and Surfaces. B, Biointerfaces
Ee-Seul KangTae-Hyung Kim

Abstract

Graphene derivatives are highly promising materials for use in stem-cell-based regenerative therapies, particularly for bone regeneration. Herein, we report a graphene oxide (GO)-based hybrid platform (GOHP) that is highly effective for guiding the osteogenesis of human adipose-derived mesenchymal stem cells (hAMSCs). A GO-coated indium tin oxide (ITO) substrate was electrochemically modified with Au nanostructures (GNSs), following which a cysteine-modified quadruple-branched arginine-glycine-aspartic acid was self-assembled on the ITO-GO-GNS hybrid via Au-S bonds. The synthesized GOHP, with the highest density of GNSs (deposition time of 120 s), exhibited the highest osteogenic differentiation efficiency based on the osteogenic marker expression level, osteocalcin expression, and osteoblastic mineralisation. Remarkably, although GO is known to be less efficient than the high-quality pure graphene synthesised via chemical vapour deposition (CVD), the fabricated GOHP exhibited an efficiency similar to that of CVD-grown graphene in guiding the osteogenesis of hAMSCs. The total RNA sequencing results revealed that CVD graphene and GOHP induced the osteogenesis of hAMSCs by upregulating the transcription factors related to direct ...Continue Reading

References

Aug 6, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Vonda WrightJohnny Huard
Aug 9, 2002·Molecular and Cellular Biology·Barbara K Brott, Sergei Y Sokol
Aug 19, 2003·Biomaterials·Ulrich HerselHorst Kessler
Jul 9, 2004·Biochemical and Biophysical Research Communications·Nitaya IndrawattanaAhnond Bunyaratvej
Sep 4, 2004·Stem Cells and Development·Adel Alhadlaq, Jeremy J Mao
Jul 21, 2006·Nature·Sasha StankovichRodney S Ruoff
Aug 16, 2006·European Journal of Nuclear Medicine and Molecular Imaging·Ingrid DijkgraafOtto C Boerman
Sep 9, 2006·Reviews in Endocrine & Metabolic Disorders·Peter V N Bodine, Barry S Komm
Jul 26, 2008·Nature Nanotechnology·Dan LiGordon G Wallace
Jan 16, 2009·Molecular Biology of the Cell·Matthew D BuschmanSara A Courtneidge
Jun 23, 2009·Science·A K Geim
Apr 30, 2010·Science Translational Medicine·Steven MinearJill A Helms
Aug 14, 2010·Advanced Materials·Yanwu ZhuRodney S Ruoff
Aug 17, 2010·Biosensors & Bioelectronics·Md Abdul KafiJeong-Woo Choi
Nov 26, 2010·Nature Chemistry·Kian Ping LohManish Chhowalla
Aug 9, 2011·Advanced Materials·Sung Young ParkSeunghun Hong
Apr 20, 2012·ACS Nano·Qiaoliang Bao, Kian Ping Loh
May 29, 2012·Nature Materials·Britta TrappmannWilhelm T S Huck
Aug 7, 2012·Biomaterials·Omid AkhavanAlireza Akhavan
Oct 13, 2012·Nature·K S NovoselovK Kim
Jan 30, 2013·Biomaterials·Xuan WangJiandong Ding
Feb 12, 2013·Nanoscale·Adriano AmbrosiMartin Pumera
May 16, 2013·Journal of Cellular and Molecular Medicine·Yang-Kao Wang, Christopher S Chen
Jul 11, 2013·Nature Reviews. Molecular Cell Biology·Fiona M Watt, Wilhelm T S Huck
Sep 26, 2013·ACS Nano·Morteza MahmoudiWolfgang J Parak
Jun 9, 2016·Biosensors & Bioelectronics·I Mitch TaylorXinyan Tracy Cui
Feb 14, 2017·Nano Convergence·Ee-Seul KangTae-Hyung Kim
Apr 4, 2017·Journal of Orthopaedics·Vi NguyenAaron W James
Sep 5, 2017·Biochemical and Biophysical Research Communications·Intan Rosalina SuhitoTae-Hyung Kim
May 11, 2018·Colloids and Surfaces. B, Biointerfaces·Ee-Seul KangTae-Hyung Kim

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