Enhancing phytochemical levels, enzymatic and antioxidant activity of spinach leaves by chitosan treatment and an insight into the metabolic pathway using DART-MS technique

Food Chemistry
Shachi Singh

Abstract

Phytochemicals are health promoting compounds, synthesized by the plants to protect them against biotic or abiotic stress. The metabolic pathways leading to the synthesis of these phytochemicals are highly inducible; therefore methods could be developed to enhance their production by the exogenous application of chemical inducers/elicitors. In the present experiment, chitosan was used as an elicitor molecule to improve the phytochemical content of spinach plant. When applied at a concentration of 0.01 mg/ml as a foliar spray, chitosan was able to cause an increase in the enzymatic (peroxidase, catalase and phenylalanine ammonium lyase (PAL)) and non enzymatic (total phenolics, flavonoids and proteins) defensive metabolites, as well as, in the total antioxidant activity of the spinach leaves. A 1.7-fold increase in the total phenolics, a 2-fold increase in total flavonoid and a 1.6-fold increase in total protein were achieved with the treatment. A higher level of enzymatic activity was observed with a 4-fold increase in peroxidase and approximately 3-fold increases in catalase and phenylalanine ammonium lyase activity. Antioxidant activity showed a positive correlation between phenolic compounds and the enzymatic activity. Direc...Continue Reading

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Citations

Jun 28, 2016·International Journal of Molecular Sciences·Massimo Malerba, Raffaella Cerana
Dec 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Carla PereiraIsabel C F R Ferreira
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Dec 22, 2020·Journal of the Science of Food and Agriculture·Samaneh Kahromi, Jalil Khara
Jun 3, 2021·Biomolecules·Mohamad Hesam ShahrajabianSpyridon A Petropoulos

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