Enhancing polyphenol extraction from unripe apples by carbohydrate-hydrolyzing enzymes.

Journal of Zhejiang University. Science. B
Hu-zhe ZhengShin-Kyo Chung

Abstract

The effects of process variables such as enzyme types, enzyme ratio, reaction temperature, pH, time, and ethanol concentration on the extraction of unripe apple polyphenol were investigated. The results indicated that Viscozyme L had the strongest effect on polyphenols extraction and was selected to study the polyphenol composition. The ratio of enzyme (Viscozyme L) to substrate (2 fungal beta-glucanase units (FBG)) at 0.02, reaction at pH 3.7, 50 degrees C for 12 h, and ethanol concentration of 70% were chosen as the most favorable extraction condition. Total phenolic content (TPC), reducing sugar content (RSC), and extraction yield increased by about 3, 1.5, and 2 times, respectively, compared with control. The contents of p-coumaric acid, ferulic acid, and caffeic acid increased to 8, 4, and 32 times, respectively. The enzyme-aided polyphenol extraction process from unripe apples might be applied to food industry for enhancing bioactive compound production.

References

Nov 23, 2000·Journal of Agricultural and Food Chemistry·A YanagidaJ G Oliveira Cordeiro
Jul 17, 2001·Journal of Agricultural and Food Chemistry·A K Landbo, A S Meyer
Aug 24, 2001·International Journal of Biological Macromolecules·C M RenardJ F Drilleau
Mar 17, 2005·Journal of Agricultural and Food Chemistry·Dejian HuangRonald L Prior
May 16, 2006·Carbohydrate Research·Isabelle BenoitMichèle Asther
Apr 3, 2007·Phytochemistry·Catherine M G C RenardPascale Guillermin
Oct 27, 2007·Journal of Agricultural and Food Chemistry·Stefania D'AngeloPatrizia Galletti
Dec 12, 2007·Journal of Separation Science·Constantine D Stalikas
Dec 15, 2007·Journal of Agricultural and Food Chemistry·Dongxiao Sun-WaterhouseBronwen G Smith

❮ Previous
Next ❯

Citations

Jul 6, 2014·Journal of Agricultural and Food Chemistry·Nguyen Thai HuynhKatleen Raes
Jan 22, 2014·Food Chemistry·Beatriz A Acosta-EstradaSergio O Serna-Saldívar
Sep 15, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fereidoon Shahidi, Ju-Dong Yeo
Oct 5, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lu WangZhenqiang Wu
Jan 12, 2019·Critical Reviews in Food Science and Nutrition·Minwei XuBingcan Chen
Dec 4, 2019·Journal of Food Biochemistry·Byoung Ok ChoSeon Il Jang
Mar 8, 2019·BMC Complementary and Alternative Medicine·Sang-Hyuk JungChang-Seon Myung
Feb 25, 2020·Journal of Agricultural and Food Chemistry·Zhenyu WangShaoling Lin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.