Enhancing PUFA-rich polar lipids in Tisochrysis lutea using adaptive laboratory evolution (ALE) with oscillating thermal stress.

Applied Microbiology and Biotechnology
Manon GachelinAntoine Sciandra

Abstract

Adaptive laboratory evolution is a powerful tool for microorganism improvement likely to produce enhanced microalgae better tailored to their industrial uses. In this work, 12 wild-type strains of Tisochrysis lutea were co-cultivated under increasing thermal stress for 6 months. Indeed, temperature was oscillating daily between a high and a low temperature, with increasing amplitude along the experiment. The goal was to enhance the polyunsaturated fatty acid content of the polar lipids. Samples were taken throughout the evolution experiment and cultivated in standardized conditions to analyze the evolution of the lipid profile. Genomic analysis of the final population shows that two strains survived. The lipid content doubled, impacting all lipid classes. The fatty acid analyses show a decrease in SFAs correlated with an increase in monounsaturated fatty acids (MUFAs), while changes in polyunsaturated fatty acid (PUFAs) vary between both photobioreactors. Hence, the proportion of C18-MUFAs (18:1 n-9) and most C18-PUFAs (18:2 n-6, 18:3 n-3, and 18:4 n-3) increased, suggesting their potential role in adjusting membrane fluidity to temperature shifts. Of particular interest, DHA in polar lipids tripled in the final population whil...Continue Reading

References

Mar 30, 2006·Journal of Bioscience and Bioengineering·Pauline SpolaoreArsène Isambert
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Rosamond L NaylorPeter D Nichols
Jul 3, 2013·Microbial Cell Factories·Martin Dragosits, Diethard Mattanovich
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Sep 22, 2016·Applied Microbiology and Biotechnology·N F Santos-SánchezR Salas-Coronado
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