Enhancing sensitivity of human herpes virus diagnosis with DNA microarrays using dendrimers

Experimental and Molecular Pathology
Hans-Martin StriebelAxel Stelzner

Abstract

DNA microarray technology has become a promising new tool for the detection and identification of viral pathogens in human plasma and cell cultures. For exploration of this technology, we have developed DNA microarrays that encode capture oligonucleotide probes for different human herpes viruses: herpes simplex virus (HSV) HSV-1, HSV-2, varicella zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), and HHV-6. The on-chip hybridization is accomplished with the PCR amplicons of the respective human herpes virus types. In this original article, we attached multiple Cy3-fluorophores to the branched 5' ends of the labeling oligonucleotide primers. For the first time, we experimentally demonstrated that the self-designed, knowledge-based, and focused microarrays specifically hybridized to fluorophore-labeled pathogenic DNAs using dendrimer technology. The fluorescence signal enhancement via the dendrimers was up to 30 times compared with the quenched single Cy3-fluorophore-labeled HSV-1 DNA. The on-chip signal-amplifying effect depended upon the number of branches and the concentration of fluorophore-labeled pathogenic DNAs. Treblers were superior to doublers, as trebler-labeled nucleic acids had fluorescence-signal-e...Continue Reading

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Citations

Dec 24, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Siddhesh B ShindeVandana B Patravale
Mar 17, 2010·Ultramicroscopy·So Yeon KimJunhong Min
Aug 2, 2006·Transfusion Medicine·J Petrik
Jan 20, 2009·IEEE Engineering in Medicine and Biology Magazine : the Quarterly Magazine of the Engineering in Medicine & Biology Society·Tristan BarrettHisataka Kobayashi
May 16, 2006·Trends in Biotechnology·Jin-Woo Bae, Yong-Ha Park
Feb 7, 2006·Biophysical Chemistry·Young Shin ChoiSeog K Kim
Nov 22, 2005·Experimental and Molecular Pathology·Masahiro FujimuroHideyoshi Yokosawa
Oct 31, 2014·Journal of Clinical Microbiology·Miguel A MartínezPavel Isa
Nov 30, 2005·BioTechniques·Concepcion Conejero-GoldbergE Fuller Torrey

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