Enhancing sensitivity of liquid chromatographic/ion-trap tandem mass spectrometric determination of estrogens by on-line pre-column derivatization

Journal of Chromatography. a
David Matejícek, Vlastimil Kubán

Abstract

A rapid and sensitive liquid chromatographic/ion-trap mass spectrometric method has been developed for the simultaneous determination of estriol, alpha-, beta-estradiol, 17alpha-ethynylestradiol and estrone. The substances were extracted from river water samples (typically 250 ml) and cleaned-up using two-stage solid-phase extraction (SPE). 12-(Difluoro-1,3,5-triazinyl)-benz[f]isoindolo-[1,2b][1,3]benzothiazolidine was applied as a derivatizing agent. The recovery for each compounds ranged from 75 to 88% and the repeatability represented as RSDs ranged from 5.6 to 8.4%. Limits of detection (LOD, 3 x S/N) were 67-285 pg/l.

References

Jan 5, 2002·Fresenius' Journal of Analytical Chemistry·M J López de Alda, D Barceló
May 26, 2007·Rapid Communications in Mass Spectrometry : RCM·Ying-Hsuan LinGen-Shuh Wang

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Citations

Feb 20, 2016·Analytical and Bioanalytical Chemistry·Kun WangZiwei Zhang
Jan 26, 2016·Journal of Chromatography. a·Marcello LocatelliFausto Croce
Dec 7, 2010·Journal of Pharmaceutical and Biomedical Analysis·Sándor Görög
Sep 12, 2015·Environmental Monitoring and Assessment·Priscilla Garozi ZanchettaAngelina Pena

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