Enhancing the accumulation of omega-3 long chain polyunsaturated fatty acids in transgenic Arabidopsis thaliana via iterative metabolic engineering and genetic crossing.

Transgenic Research
Noemi Ruiz-LopezOlga Sayanova

Abstract

The synthesis and accumulation of long chain polyunsaturated fatty acids such as eicosapentaenoic acid has previously been demonstrated in the seeds of transgenic plants. However, the obtained levels are relatively low, indicating the need for further studies and the better definition of the interplay between endogenous lipid synthesis and the non-native transgene-encoded activities. In this study we have systematically compared three different transgenic configurations of the biosynthetic pathway for eicosapentaenoic acid, using lipidomic profiling to identify metabolic bottlenecks. We have also used genetic crossing to stack up to ten transgenes in Arabidopsis. These studies indicate several potential approaches to optimize the accumulation of target fatty acids in transgenic plants. Our data show the unexpected channeling of heterologous C20 polyunsaturated fatty acids into minor phospholipid species, and also the apparent negative metabolic regulation of phospholipid-dependent Δ6-desaturases. Collectively, this study confirms the benefits of iterative approaches to metabolic engineering of plant lipid synthesis.

References

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Citations

Jan 28, 2014·PloS One·James R PetrieSurinder P Singh
Feb 14, 2015·International Journal of Molecular Sciences·Kathleen L Hefferon
Feb 24, 2016·European Journal of Lipid Science and Technology : EJLST·Johnathan A NapierOlga Sayanova
Oct 17, 2012·Plant Biotechnology Journal·Richard P HaslamJohnathan A Napier
Mar 13, 2014·Current Opinion in Biotechnology·T Catalina Adarme-VegaPeer M Schenk
May 9, 2014·Current Opinion in Plant Biology·Johnathan A NapierEdgar B Cahoon
Nov 25, 2014·Applied Microbiology and Biotechnology·Noemi Ruiz-LopezRichard P Haslam
Aug 3, 2016·The Plant Journal : for Cell and Molecular Biology·Richard P HaslamJohnathan A Napier
Mar 21, 2017·BioMed Research International·Waleed Amjad KhanFarooq Shah
Jul 14, 2021·Current Nutrition Reports·Bharat KapoorSavita Bhardwaj
Aug 7, 2021·Frontiers in Nutrition·Michela Cerone, Terry K Smith

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