PMID: 15329279Aug 27, 2004Paper

Enhancing the repair quality of skin injury on porcine after autografting with the bone marrow mesenchymal stem cells

Zhonghua yi xue za zhi
Xiaobing FuBiao Cheng

Abstract

To explore the effects of bone marrow mesenchymal stem cells (MSCs) on the quality of healing of porcine skin wounds after burn injury so as to provide a new method for clinical skin repair in the future. Seventy-two deep-partial thickness burn wounds were produced on the back of 6 minipigs and then the pigs were randomly divided into 6 groups: saline control, MSCs treatment, MSCs plus basic fibroblast growth factor (bFGF) treatment, MSCs plus epidermal growth factor (EGF) treatment, bFGF treatment or EGF treatment only. MSCs were isolated from porcine marrow and cultured in vitro. After labeling with BrdU, MSCs were autografted onto the skin wounds. 7, 14, 21 and 42 days after the injury, the area of the wounds were measured and the histological examination was performed to evaluate the velocity and quality of wound healing.1, 2 and 4 weeks after transplantation, immunohistochemical examinations were carried out to detect the positive staining of BrdU, cytokeratin and S-100 to evaluate the wound healing quality. The area of wounds was decreased at day 7 and most of these wounds were healed on day 21 after injury. Although there was no significant statistical difference in the contraction rate among these six groups, however, t...Continue Reading

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