Enhancing the Sensitivity of Biotinylated Surfaces by Tailoring the Design of the Mixed Self-Assembled Monolayer Synthesis

ACS Omega
Davide BlasiLuisa Torsi

Abstract

Thiolated self-assembled monolayers (SAMs) are typically used to anchor on a gold surface biomolecules serving as recognition elements for biosensor applications. Here, the design and synthesis of N-(2-hydroxyethyl)-3-mercaptopropanamide (NMPA) in biotinylated mixed SAMs is proposed as an alternative strategy with respect to on-site multistep functionalization of SAMs prepared from solutions of commercially available thiols. In this study, the mixed SAM deposited from a 10:1 solution of 3-mercaptopropionic acid (3MPA) and 11-mercaptoundecanoic acid (11MUA) is compared to that resulting from a 10:1 solution of NMPA:11MUA. To this end, surface plasmon resonance (SPR) and attenuated total reflectance infrared (ATR-IR) experiments have been carried out on both mixed SAMs after biotinylation. The study demonstrated how the fine tuning of the SAM features impacts directly on both the biofunctionalization steps, i.e., the biotin anchoring, and the biorecognition properties evaluated upon exposure to streptavidin analyte. Higher affinity for the target analyte with reduced nonspecific binding and lower detection limit has been demonstrated when NMPA is chosen as the more abundant starting thiol. Molecular dynamics simulations complemen...Continue Reading

References

Jan 1, 1990·Methods in Enzymology·J P Kraehenbuhl, C Bonnard
Nov 1, 1989·Trends in Pharmacological Sciences·R Barlow, J F Blake
Oct 6, 2000·Methods : a Companion to Methods in Enzymology·W M MullettJ M Yeung
Apr 28, 2001·Annual Review of Physical Chemistry·D K Schwartz
Apr 14, 2005·Chemical Reviews·J Christopher LoveGeorge M Whitesides
Oct 14, 2005·Journal of Computational Chemistry·James C PhillipsKlaus Schulten
Aug 12, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Ryan J WhiteKevin W Plaxco
Oct 19, 2010·Biosensors & Bioelectronics·Abdennour AbbasQuan Cheng
Jan 26, 2011·Chemical Society Reviews·Debasis Samanta, Amitabha Sarkar
Jun 15, 2011·Biosensors & Bioelectronics·Cesar Rodriguez-EmmeneggerAldo Bologna Alles
Apr 3, 2012·Journal of Biophotonics·Xiaowei Guo
Jun 9, 2012·Molecular Immunology·Karolina A MajorekWladek Minor
Jul 3, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Hirokazu SetoYoshiko Miura
Feb 25, 2014·Colloids and Surfaces. B, Biointerfaces·Erin E BedfordClaire-Marie Pradier
Jan 17, 2015·Nature Communications·Mohammad Yusuf MullaLuisa Torsi
Apr 21, 2016·ACS Nano·John C ThomasPaul S Weiss
Jan 31, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Norman LeoXiangqun Zeng
Feb 25, 2017·Journal of the American Chemical Society·Galina V DubachevaRalf P Richter
Jul 5, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Baozhen WangJun-Ichi Anzai
Aug 15, 2018·Nature Communications·Eleonora MacchiaLuisa Torsi
Mar 30, 2019·Analytical and Bioanalytical Chemistry·Eleonora MacchiaLuisa Torsi
Dec 23, 2019·Analytical and Bioanalytical Chemistry·Eleonora MacchiaLuisa Torsi
May 15, 2020·ACS Materials Letters·Kevin M CheungPaul S Weiss

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Citations

Dec 4, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zenonas KuodisGediminas Niaura

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Methods Mentioned

BETA
biosensing
biosensors
biosensor
surface plasmon resonance
chips
scanning
surface spectroscopic techniques

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