Enhancing the Sensitivity of DNA and Aptamer Probes in the Dextran/PEG Aqueous Two-Phase System.

Analytical Chemistry
Qiaoshu ChenJuewen Liu

Abstract

Increasing the local concentration of DNA-based probes is a convenient way to improve the sensitivity of biosensors. Instead of using organic solvents or ionic liquids that phase-separate with water based on hydrophobic interactions, we herein studied a classic aqueous two-phase system (ATPS) comprising polyethylene glycol (PEG) and dextran. Polymers of higher molecular weights and higher concentrations favored phase separation. DNA oligonucleotides are selectively enriched in the dextran-rich phase unless the pH was increased to 12. A higher volume ratio of PEG-to-dextran and a higher concentration of PEG also enrich more DNA probes in the dextran-rich phase. The partition efficiency of the T15 DNA was enriched around seven times in the dextran phase when the volume ratio of dextran and PEG reached 1:10. The detection of limit improved by 3.6-fold in a molecular beacon-based DNA detection system with the ATPS. The ATPS also increased the sensitivity for the detection of Hg2+ and adenosine triphosphate, although these target molecules alone distributed equally in the two phases. This work demonstrates a simple method using water soluble polymers to improve biosensors.

References

May 17, 1996·Journal of Chromatography. B, Biomedical Applications·P G LantzP Rådström
Nov 28, 2001·Molecular Biotechnology·R Hatti-Kaul
Feb 16, 2006·Journal of the American Chemical Society·Yoko MiyakeAkira Ono
Jul 15, 2011·Journal of Chromatography. a·Juan A Asenjo, Barbara A Andrews
Apr 4, 2012·ACS Applied Materials & Interfaces·Youssef HelwaJuewen Liu
Apr 13, 2012·Journal of Chromatography. a·Juan A Asenjo, Barbara A Andrews
Oct 24, 2012·Nature Chemistry·Christopher A StrulsonPhilip C Bevilacqua
Jul 29, 2015·Biosensors & Bioelectronics·Mahmoud Amouzadeh TabriziLeila Farzin
Aug 15, 2015·Scientific Reports·Hyunwoo ShinJaesung Park
Sep 12, 2015·ACS Applied Materials & Interfaces·Ehsan AtefiHossein Tavana
Dec 31, 2015·Analytical and Bioanalytical Chemistry·Taiping QingYanli Lei
May 18, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Thiago Pajeú NascimentoAttilio Converti
Nov 4, 2016·Biological Procedures Online·Mujahid IqbalZonghui Yuan
Nov 15, 2017·Journal of the American Chemical Society·Alexander RoloffNathan C Gianneschi
Nov 25, 2017·Chembiochem : a European Journal of Chemical Biology·Min OuKemin Wang
Dec 28, 2017·Advanced Healthcare Materials·Alyne G TeixeiraJohn P Frampton
Apr 27, 2018·Analytical Chemistry·Baoyin YuanKemin Wang
Sep 6, 2018·Journal of Food Science·Nurhayatie SajaliMohd Nasir Mohd Desa
Jun 30, 2019·Nature Communications·Bernd EnsingSander Woutersen
Nov 22, 2019·Chemical Society Reviews·Youchuang Chao, Ho Cheung Shum
Dec 5, 2019·Journal of the American Chemical Society·Ying XiongYi Lu
Apr 22, 2020·Annual Review of Physical Chemistry·Gregory L DignonJeetain Mittal
Aug 22, 2020·Chemical Communications : Chem Comm·Jiaoli WangKemin Wang
Oct 21, 2020·Environmental Science & Technology·Kunfu PiPhilippe Van Cappellen

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Citations

Oct 20, 2021·Chemistry, an Asian Journal·Yuqing LiJuewen Liu

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