Enhancing the sensitivity of ovarian cancer cells to olaparib via microRNA-20b-mediated cyclin D1 targeting.

Experimental Biology and Medicine
Qian ZhongHanshuo Yang

Abstract

We previously reported that cyclin D1 silencing interferes with RAD51 accumulation and increases the sensitivity of BRCA1 wild-type ovarian cancer cells to olaparib. However, the mechanisms associated with cyclin D1 overexpression in ovarian cancer are not fully understood. TargetScan predicted the potential binding sites for microRNA-20b (miR-20b) and the 3'-untranslated region of cyclin D1 mRNA; thus, we used luciferase reporter assay to verify those binding sites. The Kaplan-Meier method and log-rank test were used to examine the relationship between miR-20b and progression-free survival of ovarian cancer patients in The Cancer Genome Atlas (n = 367) dataset. In vitro experiments were performed to evaluate the effects of miR-20b on cyclin D1 expression, cell cycle and response to olaparib. A peritoneal cavity metastasis model of ovarian cancer was established to determine the effect of miR-20b on the sensitivity of olaparib. Immunohistochemistry was performed to evaluate molecular mechanisms. In this work, we demonstrated that miR-20b down-regulates cyclin D1, increases the sensitivity of ovarian cancer cells to olaparib, reduces the expression of RAD51, and induces cell cycle arrest in G0/G1 phase. Ovarian cancer patients w...Continue Reading

References

May 12, 2004·Journal of Molecular Biology·Andrea Tanzer, Peter F Stadler
Aug 7, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Anish BaliSusan M Henshall
Aug 27, 2005·Cell·Shveta BaggaAmy E Pasquinelli
Oct 31, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sven RottenbergJos Jonkers
Jan 15, 2009·Cancer Research·Meredith E CrosbyPeter M Glazer
Apr 21, 2009·Nature Structural & Molecular Biology·Ashish LalDipanjan Chowdhury
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Hailiang HuRichard A Gatti
Apr 22, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Peter C FongStan B Kaye
Jun 23, 2011·Molecular Cancer Research : MCR·Yemin WangToshiyasu Taniguchi
Jul 2, 2011·Nature·UNKNOWN Cancer Genome Atlas Research Network
Jan 10, 2012·Biochemical Pharmacology·Zhengguan YangMichael D Wyatt
Oct 30, 2012·Cancer Discovery·Janneke E JaspersSven Rottenberg
Apr 4, 2014·Molecular Cancer Therapeutics·Petar Jelinic, Douglas A Levine
Oct 17, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Joyce Liu, Ursula A Matulonis
Mar 18, 2015·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Clare L ScottScott H Kaufmann
Jul 19, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Geoffrey KimRichard Pazdur
Oct 16, 2015·Cancer Discovery·Panagiotis A KonstantinopoulosAlan D D'Andrea
Jul 23, 2016·Nature·Arnab Ray ChaudhuriAndré Nussenzweig
Nov 3, 2016·Thyroid : Official Journal of the American Thyroid Association·Shubin HongHaipeng Xiao
Feb 18, 2017·Nature Reviews. Drug Discovery·Rajesha Rupaimoole, Frank J Slack
Sep 15, 2017·Oncotarget·Yue ZhangYue-Hua Jiang
Oct 5, 2017·Nature·Weixing ZhaoPatrick Sung

❮ Previous
Next ❯

Software Mentioned

Image J
TargetScan
SPSS
R

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.