PMID: 9171395Jun 1, 1997Paper

Enhancing transcription through the Escherichia coli hemolysin operon, hlyCABD: RfaH and upstream JUMPStart DNA sequences function together via a postinitiation mechanism

Journal of Bacteriology
J A Leeds, R A Welch

Abstract

Escherichia coli hlyCABD operons encode the polypeptide component (HlyA) of an extracellular cytolytic toxin as well as proteins required for its acylation (HlyC) and sec-independent secretion (HlyBD). The E. coli protein RfaH is required for wild-type hemolysin expression at the level of hlyCABD transcript elongation (J. A. Leeds and R. A. Welch, J. Bacteriol. 178:1850-1857, 1996). RfaH is also required for the transcription of wild-type levels of mRNA from promoter-distal genes in the rfaQ-K, traY-Z, and rplK-rpoC gene clusters, supporting the role for RfaH in transcriptional elongation. All or portions of a common 39-bp sequence termed JUMPStart are present in the untranslated regions of RfaH-enhanced operons. In this study, we tested the model that the JUMPStart sequence and RfaH are part of the same functional pathway. We examined the effect of JUMPStart deletion mutations within the untranslated leader of a chromosomally derived hlyCABD operon on hly RNA and HlyA protein levels in either wild-type or rfaH null mutant E. coli. We also provide in vivo physical evidence that is consistent with RNA polymerase pausing at the wild-type JUMPStart sequences.

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Citations

May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·S GottesmanK M Wassarman
Sep 13, 2005·Annual Review of Microbiology·Nadim Majdalani, Susan Gottesman
Feb 3, 2015·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Monali NandyMazumdar, Irina Artsimovitch
May 3, 2018·Nucleic Acids Research·Beth A BoudreauRobert Landick
Oct 27, 2004·Molecular Genetics and Genomics : MGG·D Vicari, I Artsimovitch
Feb 28, 2019·MBio·Irina Artsimovitch, Stefan H Knauer
Oct 17, 2017·Applied and Environmental Microbiology·Sakshi KhosaLutz Schmitt
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·I Artsimovitch, R Landick
Jul 2, 2003·FEMS Immunology and Medical Microbiology·Heinz P Hahn, Bernd Ulrich von Specht
Aug 25, 2015·Biochimica Et Biophysica Acta·Laura C Ristow, Rodney A Welch
Feb 15, 2000·Research in Microbiology·K E Rudd
Nov 1, 2005·EcoSal Plus·Rodney A Welch
Jun 24, 2016·Microbiology Spectrum·Rodney A Welch
Jan 1, 2006·EcoSal Plus·Jay L Mellies, Alex M S Barron
May 4, 2013·Chemical Reviews·Sushil Kumar Tomar, Irina Artsimovitch

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