Enhancing UDP-Rhamnose Supply for Rhamnosylation of Flavonoids in Escherichia coli by Regulating the Modular Pathway and Improving NADPH Availability.

Journal of Agricultural and Food Chemistry
Na GuJianjun Pei

Abstract

UDP-rhamnose is the main type of sugar donor and endows flavonoids with special activity, selectivity, and pharmacological properties by glycosylation. In this study, several UDP-glucose synthesis pathways and UDP-rhamnose synthases were screened to develop an efficient UDP-rhamnose biosynthesis pathway in Escherichia coli. Maximal UDP-rhamnose production reached 82.2 mg/L in the recombinant strain by introducing the cellobiose phosphorolysis pathway and Arabidopsis thaliana UDP-rhamnose synthase (AtRHM). Quercitrin production of 3522 mg/L was achieved in the recombinant strain by coupling the UDP-rhamnose generation system with A. thaliana rhamnosyltransferase (AtUGT78D1) to recycle UDP-rhamnose. To further increase UDP-rhamnose supply, an NADPH-independent fusion enzyme was constructed, the UTP supply was improved, and NADPH regenerators were overexpressed in vivo. Finally, by optimizing the bioconversion conditions, the highest quercitrin production reached 7627 mg/L with the average productivity of 141 mg/(L h), which is the highest yield of quercitrin and efficiency of UDP-rhamnose supply reported to date in E. coli. Therefore, the method described herein for the regeneration of UDP-rhamnose from cellobiose may be widely u...Continue Reading

References

Dec 15, 2000·Current Opinion in Structural Biology·M F Giraud, J H Naismith
Sep 22, 2001·The American Journal of Clinical Nutrition·R J NijveldtP A van Leeuwen
Jul 1, 1995·The Plant Cell·R. A. Dixon, N. L. Paiva
May 3, 2003·The EMBO Journal·Caryn E Outten, Valeria C Culotta
Apr 8, 2006·Biotechnology Progress·Zichao MaoRachel Ruizhen Chen
Feb 8, 2007·Bioscience, Biotechnology, and Biochemistry·Mitsuru Watanabe
Mar 13, 2008·Molecular Pharmaceutics·Effendi LeonardMattheos A G Koffas
Apr 23, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ho Sik RhoJae Youl Cho
May 4, 2011·Applied Microbiology and Biotechnology·Won-Heong LeeJin-Ho Seo
Oct 12, 2012·Applied Microbiology and Biotechnology·Won-Heong LeeJin-Ho Seo
Oct 18, 2012·Journal of Agricultural and Food Chemistry·Bong-Gyu KimJoong-Hoon Ahn
Feb 20, 2013·Applied Microbiology and Biotechnology·Won-Heong LeeJin-Ho Seo
May 2, 2014·Biotechnology Advances·Jianbo XiaoMilen I Georgiev
Dec 3, 2014·Biotechnology Advances·Hui CaoJianbo Xiao
Jul 16, 2015·Critical Reviews in Food Science and Nutrition·Jianbo Xiao
Oct 13, 2016·Journal of Agricultural and Food Chemistry·Jianjun PeiYongde Yue
Nov 12, 2016·Plant Physiology and Biochemistry : PPB·Sen YinJian-Qiang Kong
May 5, 2017·Journal of Agricultural and Food Chemistry·Yu ZhangXun Li
Jul 1, 2017·Journal of Agricultural and Food Chemistry·Jianjun PeiWei Xiao
Mar 29, 2019·Journal of Agricultural and Food Chemistry·Ying ZangMattheos A G Koffas
Jul 12, 2019·Journal of Agricultural and Food Chemistry·Linan XieYuquan Xu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.