Enigmatic Gratuitous Induction of the Covalent Flavoprotein Vanillyl-Alcohol Oxidase in Penicillium simplicissimum.

Applied and Environmental Microbiology
Marco W FraaijeWillem J H van Berkel

Abstract

When Penicillium simplicissimum is grown on veratryl alcohol, anisyl alcohol, or 4-(methoxymethyl)phenol, an intracellular covalent flavin-containing vanillyl-alcohol oxidase is induced. The induction is highest (up to 5% of total protein) during the growth phase. In addition to vanillyl-alcohol oxidase, an intracellular catalase-peroxidase is induced. Induction of vanillyl-alcohol oxidase in P. simplicissimum is prevented by the addition of isoeugenol to veratryl alcohol-containing media, but growth is unaffected. The inhibitory effect of isoeugenol on induction is not observed when anisyl alcohol or 4-(methoxymethyl)phenol is used as the growth substrate. Based on the induction experiments and the degradation pathways for veratryl and anisyl alcohol, we propose that induction of vanillyl-alcohol oxidase is superfluous when P. simplicissimum is grown on these aromatic alcohols. However, the enzyme plays an essential role in the degradation of the methyl ether of p-cresol, 4-(methoxymethyl)phenol.

References

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Citations

Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·R H van Den HeuvelW J van Berkel
Aug 22, 2001·FEBS Letters·R H van den HeuvelW J van Berkel
May 23, 2007·The Journal of Biological Chemistry·Dominic P H M HeutsMarco W Fraaije
Nov 6, 2010·Applied Biochemistry and Biotechnology·Mei-Fang ZhouGuang-Ming Zeng
Dec 10, 1999·The Journal of Biological Chemistry·M W FraaijeA Mattevi
Jul 11, 2002·The Journal of Biological Chemistry·Nora TahallahAlbert J R Heck
Jan 10, 2008·Applied Microbiology and Biotechnology·Erik W van HellemondMarco W Fraaije
May 16, 2000·The Journal of Biological Chemistry·R H van den HeuvelW J van Berkel
Sep 21, 2000·FEBS Letters·R H van den HeuvelW J van Berkel
Jan 11, 2012·Applied Microbiology and Biotechnology·Remko T WinterMarco W Fraaije
Jan 24, 2002·The Journal of Biological Chemistry·Eyal ShimoniYuval Shoham
Sep 28, 2018·Microbiology and Molecular Biology Reviews : MMBR·Bastien BissaroVincent G H Eijsink
Oct 7, 2017·PLoS Computational Biology·Gudrun GygliWillem J H van Berkel

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