Enolases from fluoride-sensitive and fluoride-resistant streptococci.

Infection and Immunity
F J Bunick, S Kashket

Abstract

The enolase from a highly fluoride-sensitive strain of Streptococcus salivarius and its fluoride-resistant mutant, as well as those from strains of Streptococcus sanguis and Streptococcus mutans with intermediate and low sensitivities to fluoride have been shown to be inhibited by fluoride. Comparisons of the purified, strain-specific enzymes showed a high degree of similarity for all preparations. The Michaelis constants for the substrate 2-phosphoglycerate were 1.3 x 10(-4) to 2.4 x 10(-4) M, pH optima were 7.3 to 7.7, and Mg2+ optima were 2 mEq/liter for all. Inhibition by fluoride required the presence of inorganic phosphate and was competitive in nature, and the calculated modified inhibition indices were found to be in the range from 3.3 x 10(-14) to 5.8 x 10(-14) M4. Percent inhibitions were determined under standardized conditions (0.16 mM NaF, 2 mM MgSO4, 0.5 mM Pi, and 0.5 mM 2-phosphoglycerate) and were found to range from 53.3 to 65.9% for all of the purified enzymes. The differences do not appear to be meaningful metabolically. Inhibition was reduced to about 14% at pH 6.0. From the similarities in the behavior of the strain-specific enzymes it is concluded that the differences in the glycolytic sensitivities of th...Continue Reading

References

Apr 1, 1975·Journal of Bacteriology·F M Harold, E Spitz
Jan 1, 1978·Archives of Oral Biology·S Kashket, F J Bunick
Jan 1, 1976·Archives of Oral Biology·S Kashket, V M Rodriguez
Jan 1, 1972·Caries Research·G Cimasoni
Aug 1, 1972·Archives of Oral Biology·F BrudevoldY Bakhos
Jan 1, 1969·Archives of Oral Biology·G N Jenkins, W M Edgar
Dec 1, 1964·Analytical Biochemistry·R F ITZHAKI, D M GILL
Aug 1, 1959·Archives of Oral Biology·G N JENKINS

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Citations

May 1, 1992·Journal of Dental Research·S G Dashper, E C Reynolds
Oct 1, 1987·Journal of Dental Research·S M Brussock, T A Kral
Apr 1, 1997·Oral Microbiology and Immunology·N Guha-ChowdhuryC H Sissons
May 11, 2004·Microbiology·Alice C L LenNicholas A Jacques
Sep 2, 2009·Microbiology and Molecular Biology Reviews : MMBR·Angela H NobbsHoward F Jenkinson
Jun 11, 2020·International Journal of Environmental Research and Public Health·Yoshiaki NomuraNobuhiro Hanada
Sep 1, 1995·Canadian Journal of Microbiology·W A BelliR E Marquis
Nov 1, 1995·Canadian Journal of Microbiology·R E Marquis
Sep 1, 1987·Oral Microbiology and Immunology·K A Lau, T A Kral
Sep 1, 1995·Journal of Industrial Microbiology·R E Marquis
Jun 1, 1987·Journal of Dental Research·S V Sutton, R E Marquis
Nov 1, 1983·Journal of Dental Research·E A Thibodeau, R E Marquis
Jun 1, 1988·Oral Microbiology and Immunology·L A Jonooby, T A Kral
Jul 28, 2017·Journal of Oral Microbiology·Ying LiaoDong Mei Deng
Oct 1, 1991·Oral Microbiology and Immunology·C Van LoverenJ M Ten Cate
Feb 1, 1985·Journal of Dental Research·G R BenderR E Marquis
Nov 1, 1985·Journal of Dental Research·S Kashket, R J Preman
Sep 13, 2017·Frontiers in Microbiology·Yanling CaiDong Mei Deng
Jan 9, 2019·Frontiers in Microbiology·Ying LiaoDong Mei Deng
Jun 1, 1988·Applied and Environmental Microbiology·A Casiano-Colón, R E Marquis
Apr 1, 1985·Infection and Immunity·S Kashket, E R Kashket
Aug 1, 1986·Infection and Immunity·G R BenderR E Marquis
Aug 1, 1984·Infection and Immunity·J S van der Hoeven, H C Franken
Jul 1, 1989·Infection and Immunity·P E Chansley, T A Kral
Jan 1, 1986·Antimicrobial Agents and Chemotherapy·G R Germaine, L M Tellefson
Aug 31, 1982·Biochemistry·F J Bunick, S Kashket

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