Enriched environment prevents oxidative stress in zebrafish submitted to unpredictable chronic stress

PeerJ
Matheus MarconAngelo Piato

Abstract

The enriched environment (EE) is a laboratory housing model that emerged from efforts to minimize the impact of environmental conditions on laboratory animals. Recently, we showed that EE promoted positive effects on behavior and cortisol levels in zebrafish submitted to the unpredictable chronic stress (UCS) protocol. Here, we expanded the characterization of the effects of UCS protocol by assessing parameters of oxidative status in the zebrafish brain and reveal that EE protects against the oxidative stress induced by chronic stress. Zebrafish were exposed to EE (21 or 28 days) or standard housing conditions and subjected to the UCS protocol for seven days. Oxidative stress parameters (lipid peroxidation (TBARS), reactive oxygen species (ROS) levels, non-protein thiol (NPSH) and total thiol (SH) levels, superoxide dismutase (SOD) and catalase (CAT) activities were measured in brain homogenate. Our results revealed that UCS increased lipid peroxidation and ROS levels, while decreased NPSH levels and SOD activity, suggesting oxidative damage. EE for 28 days prevented all changes induced by the UCS protocol, and EE for 21 days prevented the alterations on NPSH levels, lipid peroxidation and ROS levels. Both EE for 21 or 28 days ...Continue Reading

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Citations

Nov 27, 2019·Naunyn-Schmiedeberg's Archives of Pharmacology·Carlos G ReisAngelo Piato
Jul 26, 2019·Environmental Science and Pollution Research International·Jéssica ValadasAngelo Piato
Apr 15, 2020·Pharmacology, Biochemistry, and Behavior·Murilo S de AbreuAllan V Kalueff
Apr 4, 2021·Animals : an Open Access Journal From MDPI·Chloe H StevensPenny Hawkins
May 5, 2021·Journal of Fish Biology·Nick A R JonesAnne Gro Vea Salvanes
Jun 21, 2021·Progress in Neuro-psychopharmacology & Biological Psychiatry·Pablo Ricardo BertelliAngelo Piato
May 15, 2020·Physiology & Behavior·Suelen Mendonça SoaresLeonardo José Gil Barcellos

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Methods Mentioned

BETA
histone acetylation

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