Enriched housing promotes post-stroke neurogenesis through calpain 1-STAT3/HIF-1α/VEGF signaling

Brain Research Bulletin
Xiaoying WuGuangzhi Wang

Abstract

Enriched environment (EE) has been shown to promote neurogenesis and functional recovery after ischemic stroke. However, the underlying molecular mechanisms are not fully understood. In this study, C57BL/6 mice underwent middle cerebral artery occlusion (60 min) followed by reperfusion, after which mice were housed in either standard environment (SE) or EE and allowed to survive for 3, 4, 6 or 10 weeks. Ipsilateral subventricular zone (SVZ) or striatum cells were dissociated from ischemic hemispheric brains of enriched mice at 14 days post-ischemia (dpi) and cultured in vitro. Our data showed that post-ischemic EE inhibited calpain 1 activity, and increased the expression of phosphorylated signal transducer and activator of transcription 3 (p-STAT3) in the ischemic hemisphere of enriched mice at 21 dpi. Calpain 1-specific inhibitor PD151746 further increased p-STAT3 expression and augmented the promoting effects of EE on post-stroke SVZ neural precursor cells (NPCs) proliferation and functional recovery. EE also increased the expression of hypoxia-inducible factor 1-alpha (HIF-1α) and vascular endothelial growth factor (VEGF) in the ischemic hemisphere at 21 dpi. Inhibition of the JAK/STAT3 pathway with AG490 decreased the expr...Continue Reading

Citations

Jul 25, 2018·Toxins·Maryam AssemLucie Hénaut
Oct 3, 2018·Biomedical Reports·Shu-Wen MuJian-Jun Gu
Mar 17, 2020·Frontiers in Immunology·Shenbin XuJianmin Zhang
Aug 31, 2020·Translational Stroke Research·Abir A RahmanGregory J Bix
Oct 22, 2020·The International Journal of Neuroscience·Na JiaLina Sun
Oct 31, 2020·Cellular and Molecular Neurobiology·Chengli LiuWei Wang
Sep 17, 2020·Immunology·Qiaohui ZhangYibo Tang
Dec 31, 2020·International Journal of Molecular Sciences·Aleah HolmesVenugopal Reddy Venna
Jul 3, 2021·Frontiers in Molecular Biosciences·Michel BaudryXiaoning Bi

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