Enrichment and characteristics of mixed methane-oxidizing bacteria from a Chinese coal mine

Applied Microbiology and Biotechnology
Hao JiangXin-Hui Xing

Abstract

In methane-rich environments, methane-oxidizing bacteria usually occur predominantly among consortia including other types of microorganisms. In this study, artificial coal bed gas and methane gas were used to enrich mixed methanotrophic cultures from the soil of a coal mine in China, respectively. The changes in microbial community structure and function during the enrichment were examined. The microbial diversity was reduced as the enrichment proceeded, while the capacity for methane oxidation was significantly enhanced by the increased abundance of methanotrophs. The proportion of type II methanotrophs increased greatly from 7.84 % in the sampled soil to about 50 % in the enrichment cultures, due to the increase of methane concentration. After the microbial community of the cultures got stable, Methylomonas and Methylocystis became the dominant type I and type II methanotrophs, while Methylophilus was the prevailing methylotroph. The sequences affiliated with pigment-producing strains, Methylomonas rubra, Hydrogenophaga sp. AH-24, and Flavobacterium cucumis, could explain the orange appearance of the cultures. Comparing the two cultures, the multi-carbon sources in the artificial coal bed gas caused more variety of non-metha...Continue Reading

References

Jan 1, 1978·Antonie van Leeuwenhoek·J S RokemI Goldberg
Jan 1, 1991·Journal of Bacteriology·W G WeisburgD J Lane
May 1, 1995·The Journal of Applied Bacteriology·D Hrsak, D Grbić-Galić
Feb 6, 2004·Environmental Microbiology·Elena HutchensJ Colin Murrell
May 4, 2005·Biodegradation·Martin HesselsoePeter Roslev
Sep 9, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Howard Dalton
Oct 22, 2005·Molecular Microbiology·Andreas R TheisenJ Colin Murrell
Jul 13, 2007·International Journal of Systematic and Evolutionary Microbiology·Hang-Yeon WeonErko Stackebrandt
Apr 18, 2008·Briefings in Bioinformatics·Sudhir KumarKoichiro Tamura
Jun 12, 2009·FEMS Microbiology Ecology·Bing HanXin-Hui Xing
Mar 20, 2010·FEMS Microbiology Reviews·Jeremy D SemrauSukhwan Yoon
Jun 19, 2010·Applied Microbiology and Biotechnology·David van der HaNico Boon
Jul 3, 2010·Bioresource Technology·Awadhesh K ShuklaSuresh K Dubey
Nov 26, 2010·International Journal of Systematic and Evolutionary Microbiology·Alexey V VorobevS N Dedysh
May 31, 2013·Environmental Microbiology·Loïc NazariesBrajesh Kumar Singh
Jun 12, 2013·Environmental Microbiology Reports·Adrian HoPaul L E Bodelier
Feb 28, 2015·Environmental Science & Technology·P J StrongW P Clarke

Citations

May 12, 2020·Frontiers in Bioengineering and Biotechnology·Meng-Ting SunRong-Bo Guo

Related Concepts

Methylococcaceae
Soil Microbiology
Biological Community
Carbon
Coal
Interferon Type I
Methane
Oxidation
Pigment
Laboratory Culture

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.