Enrichment and Characterization of a Psychrotolerant Consortium Degrading Crude Oil Alkanes Under Methanogenic Conditions

Microbial Ecology
Chen DingHui Zhang

Abstract

Anaerobic alkane degradation via methanogenesis has been intensively studied under mesophilic and thermophilic conditions. While there is a paucity of information on the ability and composition of anaerobic alkane-degrading microbial communities under low temperature conditions. In this study, we investigated the ability of consortium Y15, enriched from Shengli oilfield, to degrade hydrocarbons under different temperature conditions (5-35 °C). The consortium could use hexadecane over a low temperature range (15-30 °C). No growth was detected below 10 °C and above 35 °C, indicating the presence of cold-tolerant species capable of alkane degradation. The preferential degradation of short chain n-alkanes from crude oil was observed by this consortium. The structure and dynamics of the microbial communities were examined using terminal restriction fragment length polymorphism (T-RFLP) fingerprinting and Sanger sequencing of 16S rRNA genes. The core archaeal communities were mainly composed of aceticlastic Methanosaeta spp. Syntrophaceae-related microorganisms were always detected during consecutive transfers and dominated the bacterial communities, sharing 94-96 % sequence similarity with Smithella propionica strain LYP(T). Phyloge...Continue Reading

References

Jul 2, 1998·Applied and Environmental Microbiology·L G WhyteC W Greer
Aug 25, 2001·Science·D R Lovley
Oct 17, 2001·Applied Microbiology and Biotechnology·R Margesin, F Schinner
May 31, 2002·Nature·Jay A Labinger, John E Bercaw
Dec 6, 2003·Environmental Science & Technology·G Todd TownsendJoseph M Suflita
Apr 10, 2004·Bioinformatics·Thomas HuberPhilip Hugenholtz
May 23, 2006·Nature Reviews. Microbiology·Ricardo Cavicchioli
Aug 4, 2007·Applied Microbiology and Biotechnology·Alexander WentzelMimmi Throne-Holst
Dec 15, 2007·The ISME Journal·Jan DolfingIan M Head
Mar 21, 2008·Journal of Hazardous Materials·Huabo DuanJinhui Li
Apr 2, 2008·Applied and Environmental Microbiology·Lisa M GiegJoseph M Suflita
Nov 14, 2008·Nucleic Acids Research·J R ColeJ M Tiedje
Oct 8, 2009·Environmental Microbiology·Fernando Rojo
May 28, 2010·Environmental Science & Technology·Amy V CallaghanBoris Wawrik
Jul 7, 2010·Environmental Microbiology·Lisa M GiegJoseph M Suflita
Jul 27, 2010·Canadian Journal of Microbiology·Tara J Penner, Julia M Foght
Jan 1, 2007·CSH Protocols·David W Mount
Jun 8, 2011·Environmental Science & Technology·Tariq SiddiqueJulia M Foght
Jan 18, 2012·Applied Microbiology and Biotechnology·Serge Maurice MbadingaBo-Zhong Mu
May 31, 2012·Science China. Life Sciences·XiuZhu Dong, ZiJuan Chen
Aug 17, 2012·Environmental Science & Technology·Tariq SiddiqueJulia M Foght
Jul 3, 2013·Environmental Microbiology·Karolin TischerAnnelie Wendeberg

❮ Previous
Next ❯

Citations

Aug 24, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dan WangShuang Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Archaeogenetics

Recent advances in genomic sequencing has led to the discovery of new strains of Archaea and shed light on their evolutionary history. Discover the latest research on Archaeogenetics here.

Bioremediation (ASM)

Bioremediation is the treatment and removal of harmful pollutants or contaminants through the use of microorganisms. Discover the latest research here.