Enrichment and culture of spermatogonia from cryopreserved adult bovine testis tissue

Animal Reproduction Science
Huan CaiXue-ming Zhang

Abstract

Propagation of bovine spermatogonial stem cells (SSCs) from the cryopreserved testicular tissue is essential for the application of SSCs-related techniques. To explore the appropriate conditions for in vitro culture of bovine spermatogonia (containing putative SSCs), Sertoli cell monolayer and serum concentration were set as two main control factors. Morphological examination showed that the intactness and structure of adult bovine testicular tissue were well maintained after cryopreservation. The enriched bovine spermatogonia were large round CD9 and promyelocytic leukemia zinc finger protein (PLZF) positive cells, with high nucleocytoplasmic ratios and multiple types including single, paired-, aligned-cells or grape cluster-like colonies in vitro. In Sertoli cell co-culture system, bovine spermatogonia attached quickly and proliferated obviously faster than those in the system without Sertoli cells. Serum-free media was no good for the attachment and proliferation of bovine spermatogonia. When 2.5%, 5% and 10% fetal bovine serum (FBS) was employed in the media, spermatogonia attached easily and divided quickly to form paired-, chained-cells or grape cluster-like colonies with comparable percentages in all groups. However, the...Continue Reading

References

Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·R L Brinster, J W Zimmermann
Sep 20, 2000·Anatomy and Embryology·K H Wrobel
Jul 2, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·F IzadyarD G de Rooij
Aug 16, 2002·Nature·Ali HonaramoozStefan Schlatt
Sep 5, 2003·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
May 21, 2004·Biology of Reproduction·Jon M OatleyDerek J McLean
May 25, 2004·Nature Genetics·José A CostoyaPier Paolo Pandolfi
Dec 3, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Rahul RathiIna Dobrinski
Dec 19, 2008·Reproduction in Domestic Animals = Zuchthygiene·B XieH Jiang
Mar 6, 2009·Reproduction, Fertility, and Development·Muren HerridJonathan R Hill
Mar 6, 2009·Reproduction, Fertility, and Development·W ZengI Dobrinski
Feb 16, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Suzanne C RedingJon M Oatley
Aug 31, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Emily AnglinNicolas H Voelcker
Oct 12, 2010·Journal of Andrology·Jose R Rodriguez-SosaIna Dobrinski
Dec 14, 2011·Journal of Assisted Reproduction and Genetics·M BahadoraniMohammad H Nasr-Esfahani
Oct 9, 2012·Cell Stem Cell·Mito Kanatsu-ShinoharaTakashi Shinohara
Jun 20, 2013·Journal of Assisted Reproduction and Genetics·Yi ZhengWenxian Zeng
Sep 7, 2013·Animal Reproduction Science·Muren HerridSigrid A Lehnert
Oct 12, 2013·Journal of Cellular Physiology·Ying GuoZuping He
Nov 15, 2013·Biology of Reproduction·Catriona PaulBernard Robaire
Dec 21, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Yi ZhengWenxian Zeng
Dec 17, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Su-Ren Chen, Yi-Xun Liu
Jul 17, 2015·Reproduction, Fertility, and Development·Mahesh SahareHiroshi Imai

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Citations

Nov 13, 2020·World Journal of Stem Cells·Han-Chao LiuGui-Hua Liu
Mar 26, 2021·Andrologia·Huan CaiXue-Ming Zhang
Jul 14, 2021·Animal Science Journal = Nihon Chikusan Gakkaihō·Terumichi KawaharaKenshiro Hara

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