Enrichment and differentiation of human germ-like cells mediated by feeder cells and basic fibroblast growth factor signaling

Stem Cells
Franklin D WestSteven L Stice

Abstract

Human embryonic stem cells (hESCs) have recently demonstrated the potential for differentiation into germ-like cells in vitro. This provides a novel model for understanding human germ cell development and human infertility. Mouse embryonic fibroblast (MEF) feeders and basic fibroblast growth factor (bFGF) are two sources of signaling that are essential for primary culture of germ cells, yet their role has not been examined in the derivation of germ-like cells from hESCs. Here protein and gene expression demonstrated that both MEF feeders and bFGF can significantly enrich germ cell differentiation from hESCs. Under enriched differentiation conditions, flow cytometry analysis proved 69% of cells to be positive for DDX4 and POU5F1 protein expression, consistent with the germ cell lineage. Importantly, removal of bFGF from feeder-free cultures resulted in a 50% decrease in POU5F1- and DDX4-positive cells. Quantitative reverse transcription-polymerase chain reaction analysis established that bFGF signaling resulted in an upregulation of genes involved in germ cell differentiation with or without feeders; however, feeder conditions caused significant upregulation of premigratory/migratory (Ifitm3, DAZL, NANOG, and POU5F1) and postmig...Continue Reading

References

Nov 1, 1983·The Journal of Experimental Zoology·L Zamboni, S Upadhyay
Jun 28, 1996·Cell·W EdelmannR Kucherlapati
Jul 1, 1997·Developmental Biology·A McLaren, D Southee
Oct 22, 1998·Biology of Reproduction·J L ResnickP J Donovan
Nov 6, 1998·Science·J A ThomsonJ M Jones
Feb 27, 1999·Genes & Development·K A LawsonB L Hogan
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·D H CastrillonC P Crum
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y YingG Q Zhao
Jul 21, 2001·Stem Cells·M Pesce, H R Schöler
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 12, 2002·Nature Biotechnology·Ren-He XuJames A Thomson
May 6, 2003·Science·Karin HübnerHans R Schöler
Sep 12, 2003·Stem Cells·Maisam MitalipovaSteven Stice
Sep 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yayoi ToyookaToshiaki Noce
Sep 27, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Mitinori SaitouM Azim Surani
Feb 14, 2004·Human Molecular Genetics·Amander T ClarkRenee A Reijo Pera
May 14, 2005·Human Reproduction·Montserrat Codina-PascualJordi Benet
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jana KoubovaDavid C Page
Apr 1, 2006·Science·Josephine BowlesPeter Koopman
Sep 6, 2006·Fertility and Sterility·Elizabeth Hervey Stephen, Anjani Chandra
Sep 12, 2006·Nucleic Acids Research·Takeshi SekiguchiTakeharu Nishimoto
Jan 27, 2007·Stem Cells and Development·Kehkooi KeeRenee A Reijo Pera

❮ Previous
Next ❯

Citations

Jan 20, 2011·Stem Cells and Development·Olympia E PsathakiHans R Schöler
Feb 6, 2010·Tissue Engineering. Part B, Reviews·Debby GawlittaWouter J A Dhert
Dec 7, 2010·Human Molecular Genetics·Sarita PanulaRenee A Reijo Pera
Sep 23, 2009·Human Reproduction·B AflatoonianH D Moore
Dec 17, 2009·Journal of Molecular Cell Biology·Candace L Kerr, Linzhao Cheng
Apr 16, 2009·Endocrine Reviews·Cory R NicholasRenee A Reijo Pera
Feb 11, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Zachary Yu-Ching LinHideyuki Okano
May 3, 2014·Animal Reproduction Science·Qing-Qing ShiBi-Chun Li
Feb 19, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Jingmei HouZuping He
Feb 15, 2011·Journal of Assisted Reproduction and Genetics·Mieke GeensHerman Tournaye
Feb 22, 2013·The Malaysian Journal of Pathology·Geok Chin Tan, Nick J Dibb
Oct 27, 2011·Médecine sciences : M/S·Lucie ToscaGérard Tachdjian
Dec 2, 2010·Human Fertility : Journal of the British Fertility Society·Muhammad Lokman, Harry Moore
Jan 29, 2013·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Christine Wyns
Jan 18, 2012·Expert Opinion on Drug Metabolism & Toxicology·Francesca PistollatoGlyn Stacey
Jun 20, 2012·Systems Biology in Reproductive Medicine·Kristín Rós KjartansdóttirJens Fedder
Jun 12, 2010·Systems Biology in Reproductive Medicine·Ana Krtolica, Gnanaratnam Giritharan
Jun 5, 2012·Fertility and Sterility·Yohei HayashiShinya Yamanaka
May 14, 2010·Reproductive Biomedicine Online·Xiu-Zhen Zhang
Feb 10, 2009·Fertility and Sterility·Roberto Ensenat-WaserCarlos Simon
Oct 7, 2015·Cellular and Molecular Life Sciences : CMLS·Shigeo SaitoKazunari K Yokoyama
Oct 27, 2015·Trends in Biotechnology·Fereshteh EsfandiariHossein Baharvand
Mar 16, 2011·Annals of the New York Academy of Sciences·Emanuele PelosiDavid Schlessinger
Jun 18, 2011·Stem Cells·C EguizabalJ C Izpisua Belmonte
Sep 3, 2013·Molecular Reproduction and Development·Masanori ImamuraHideyuki Okano
Feb 14, 2016·Stem Cells and Development·Aurélie MoukaLucie Tosca
Sep 6, 2012·Current Opinion in Cell Biology·Davood Sabour, Hans R Schöler
Apr 15, 2016·Molecular Reproduction and Development·Fatemeh Sadeghian-NodoushanBehrouz Aflatoonian
Jul 15, 2015·Clinical and Experimental Reproductive Medicine·Inmaculada MorenoCarlos Simón
Mar 20, 2013·BioMed Research International·Irma Virant-KlunThomas Skutella
Mar 17, 2010·Differentiation; Research in Biological Diversity·Susana M Chuva de Sousa Lopes, Bernard A J Roelen
Dec 19, 2009·Biology of Reproduction·Amander T Clark
Feb 27, 2015·Biology of Reproduction·Fanhui ZengHu Wang
Sep 29, 2012·Biology of Reproduction·Junaid KashirKevin Coward
Apr 25, 2017·Molecular Reproduction and Development·Amir FattahiMohammad Nouri
Jun 6, 2018·Journal of Cellular Physiology·Javad Amini MahabadiHossein Nikzad

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.