Enrichment and Physiological Characterization of a Cold-Adapted Nitrite-Oxidizing Nitrotoga sp. from an Eelgrass Sediment

Applied and Environmental Microbiology
Kento IshiiSatoshi Tsuneda

Abstract

Nitrite-oxidizing bacteria (NOB) are responsible for the second step of nitrification in natural and engineered ecosystems. The recently discovered genus Nitrotoga belongs to the Betaproteobacteria and potentially has high environmental importance. Although environmental clones affiliated with Nitrotoga are widely distributed, the limited number of cultivated Nitrotoga spp. results in a poor understanding of their ecophysiological features. In this study, we successfully enriched the nonmarine cold-adapted Nitrotoga sp. strain AM1 from coastal sand in an eelgrass zone and investigated its physiological characteristics. Multistep-enrichment approaches led to an increase in the abundance of AM1 to approximately 80% of the total bacterial population. AM1 was the only detectable NOB in the bacterial community. The 16S rRNA gene sequence of AM1 was 99.6% identical to that of "Candidatus Nitrotoga arctica," which was enriched from permafrost-affected soil. The highest nitrogen oxidation rate of AM1 was observed at 16°C. The half-saturation constant (Km ) and the generation time were determined to be 25 μM NO2- and 54 h, respectively. The nitrite oxidation rate of AM1 was stimulated at concentrations of <30 mM NH4Cl but completely inh...Continue Reading

References

May 1, 1976·Anesthesiology·B F CullenL Ray-Keil
Nov 5, 1997·Advances in Microbial Physiology·J T Lin, V Stewart
Oct 19, 1999·FEMS Microbiology Reviews·R A Herbert
Dec 14, 2005·Applied and Environmental Microbiology·T A HovanecE F Delong
Jul 29, 2006·Environmental Microbiology·Frank MaixnerHolger Daims
May 8, 1964·Science·W C Fernelius
Jan 8, 2008·International Journal of Systematic and Evolutionary Microbiology·E V LebedevaE Spieck
Jul 25, 2009·International Journal of Systematic and Evolutionary Microbiology·Nadine BrulandAlexander Steinbüchel
Apr 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jennifer B H MartinyM Claire Horner-Devine
Nov 10, 2011·The Journal of Microbiology·Hyunsoo NaJongsik Chun
Jul 12, 2012·Frontiers in Microbiology·Eric E RodenJuergen Schieber
Nov 30, 2012·Nucleic Acids Research·Christian QuastFrank Oliver Glöckner
Sep 6, 2013·Microbes and Environments·Norisuke UshikiSatoshi Tsuneda
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Aug 22, 2014·Nature·Brent C ChristnerUNKNOWN WISSARD Science Team
Oct 15, 2014·Environmental Microbiology·Hirotsugu FujitaniYoshiteru Aoi
Feb 13, 2015·FEMS Microbiology Letters·Luis Sayavedra-SotoDaniel Arp
Aug 12, 2015·The ISME Journal·Aaron M SaundersPer H Nielsen

❮ Previous
Next ❯

Citations

Apr 15, 2020·Environmental Microbiology·Kento IshiiSatoshi Tsuneda
Jan 24, 2018·FEMS Microbiology Ecology·Andrew T GiguerePeter J Bottomley
Aug 17, 2019·Microbiology Resource Announcements·Hiroto IdeSatoshi Tsuneda
Aug 28, 2020·Frontiers in Microbiology·Eva SpieckAlejandra Giaveno
Aug 28, 2020·Frontiers in Microbiology·Yu TakahashiSatoshi Tsuneda
Oct 31, 2020·Water Environment Research : a Research Publication of the Water Environment Federation·Weilun ZhaoCraig Just
May 8, 2020·The Science of the Total Environment·Siqi LiMin Zheng
Sep 12, 2021·Applied Microbiology and Biotechnology·Eva SpieckSabine Keuter

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.