Enrichment and properties of a 1,2,4-trichlorobenzene-dechlorinating methanogenic microbial consortium.

Applied and Environmental Microbiology
P MiddeldorpG Schraa

Abstract

A methanogenic microbial consortium capable of reductively dechlorinating 1,2,4-trichlorobenzene (1,2,4-TCB) was enriched from a mixture of polluted sediments. 1,2,4-TCB was dechlorinated via 1,4-dichlorobenzene (1,4-DCB) to chlorobenzene (CB). Lactate, which was used as an electron donor during the enrichment, was converted via propionate and acetate to methane. Glucose, ethanol, methanol, propionate, acetate, and hydrogen were also suitable electron donors for dechlorination, whereas formate was not. The addition of 5% (wt/vol) sterile Rhine River sand was necessary to maintain the dechlorinating activity of the consortium. The addition of 2-bromoethanesulfonic acid (BrES) inhibited methanogenesis completely but had no effect on the dechlorination of 1,2,4-TCB. The consortium was also able to dechlorinate other chlorinated benzenes via various simultaneous pathways to 1,3,5-TCB, 1,2-DCB, 1,3-DCB, or CB as an end product. The addition of BrES inhibited several of the simultaneously occurring dechlorination pathways of 1,2,3,4- and 1,2,3,5-tetrachlorobenzene and of pentachlorobenzene, which resulted in the formation of CB as the only final product. Hexachlorobenzene and polychlorinated biphenyls (PCBs) were dechlorinated after ...Continue Reading

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Citations

Dec 16, 2000·Nature·L AdrianH Görisch
Oct 7, 2009·Biotechnology and Bioengineering·Didem Okutman TasSpyros G Pavlostathis
Oct 1, 2002·FEMS Microbiology Ecology·Young-Cheol ChoG-Yull Rhee
Oct 6, 2007·Biodegradation·Jim A Field, Reyes Sierra-Alvarez
Jan 5, 1999·Applied and Environmental Microbiology·F von WintzingerodeU B Göbel
May 21, 2009·Environmental Science & Technology·Jennifer M FungStephen H Zinder
Nov 4, 2010·Environmental Science & Technology·Tina HölscherLorenz Adrian
Apr 25, 2014·Environmental Science & Technology·Chunfang ZhangArata Katayama
Mar 7, 2014·Environmental Science & Technology·Jennifer L NelsonStephen H Zinder
Aug 20, 2011·Environmental Science & Technology·Xiaoming LiangBarbara Sherwood Lollar

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