Enrichment and Purification of Total Ginkgo Flavonoid O -Glycosides from Ginkgo Biloba Extract with Macroporous Resin and Evaluation of Anti-Inflammation Activities In Vitro

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Lihu ZhangLinguo Zhao

Abstract

In the present study, the performance and separation characteristics of six macroporous resins for the enrichment and purification of total ginkgo flavonoid O-glycosides (TGFs) (quercetin (I), kaempferol (II), isorhamnetin (III)) from Ginkgo Biloba extracts (EGB) are evaluated. The adsorption and desorption properties of TGFs are studied on macroporous resins, including D101, D201, AB-8, HPD400, D301, and D311. Along with the results, AB-8 resin exhibits the best adsorption and desorption capacity for these three ginkgo flavonoid O-glycosides among the six resins. Adsorption isotherms are created on AB-8 resin and fit well to the Langmuir (R² > 0.96) and Freundlich (R² > 0.92, 0.3 < 1/n < 0.7) models. After the treatment with gradient elution on AB-8 resin packed chromatography column, the contents of the three main ginkgo flavonoid O-glycosides (I, II, and III) increase from 8.93%, 9.88%, and 6.11% in the extracts to 30.12%, 35.21%, and 14.14%, respectively, in the product. The recoveries of compounds I, II, and III are 88.76%, 93.78%, and 60.90%, respectively. Additionally, the anti-inflammatory effects of TGFs are evaluated in LPS-treated RAW 264.7 macrophages, and the result demonstrates that TGFs could significantly inhibi...Continue Reading

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Citations

Feb 15, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mohamad Shazeli Che ZainKhozirah Shaari
Oct 20, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhenzhong YangXiaoping Zhao
May 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xianying FangFuliang Cao
Dec 17, 2020·Journal of Food Biochemistry·Zhipeng QiLinguo Zhao
Dec 15, 2020·The American Journal of Chinese Medicine·Da-Sol KimHye-Ock Jang
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fenfen WangShihua Shen
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ruihong LiJunxing Dong

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Methods Mentioned

BETA
equilibrium adsorption

Software Mentioned

GraphPad Prism

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