Abstract
In the present study, the performance and separation characteristics of six macroporous resins for the enrichment and purification of total ginkgo flavonoid O-glycosides (TGFs) (quercetin (I), kaempferol (II), isorhamnetin (III)) from Ginkgo Biloba extracts (EGB) are evaluated. The adsorption and desorption properties of TGFs are studied on macroporous resins, including D101, D201, AB-8, HPD400, D301, and D311. Along with the results, AB-8 resin exhibits the best adsorption and desorption capacity for these three ginkgo flavonoid O-glycosides among the six resins. Adsorption isotherms are created on AB-8 resin and fit well to the Langmuir (R² > 0.96) and Freundlich (R² > 0.92, 0.3 < 1/n < 0.7) models. After the treatment with gradient elution on AB-8 resin packed chromatography column, the contents of the three main ginkgo flavonoid O-glycosides (I, II, and III) increase from 8.93%, 9.88%, and 6.11% in the extracts to 30.12%, 35.21%, and 14.14%, respectively, in the product. The recoveries of compounds I, II, and III are 88.76%, 93.78%, and 60.90%, respectively. Additionally, the anti-inflammatory effects of TGFs are evaluated in LPS-treated RAW 264.7 macrophages, and the result demonstrates that TGFs could significantly inhibi...Continue Reading
References
Apr 1, 1992·Phytochemistry·A HaslerO Sticher
May 26, 1995·Biochemical Pharmacology·I MaitraL Packer
Mar 10, 2001·Antioxidants & Redox Signaling·T YoshikawaM Kondo
Sep 7, 2001·Biochemical Pharmacology·T L WadsworthD R Koop
Dec 31, 2003·The Journal of Surgical Research·Nazif ZeybekTurgut Tufan
Mar 22, 2007·British Journal of Pharmacology·L BiddlestoneS Dolan
Jul 22, 2008·Fitoterapia·Bikram SinghP S Ahuja
Feb 7, 2009·Journal of Chromatography. a·Teris A van Beek, Paola Montoro
Feb 12, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anna K Jäger, Lasse Saaby
Feb 22, 2012·Indian Journal of Pharmacology·Lu Ching-HsiangWu Wen-Tung
Oct 9, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zhanquan DuFeng Qiu
Feb 2, 2013·Journal of Agricultural and Food Chemistry·Amandeep K Sandhu, Liwei Gu
Jun 19, 2013·Food Chemistry·Yan SunShilin Yang
Dec 11, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Pengfei WanYanhua Li
May 31, 2014·Food Chemistry·Jiyoung KimSuengmok Cho
Oct 9, 2014·Journal of Agricultural and Food Chemistry·Nebojsa M IlicIlya Raskin
Nov 13, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Pan ZhaoXiaohong Hou
Jun 16, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yang XieGuang-Shu Wang
Jan 7, 2017·Food & Function·Jiaoyan RenWenzhen Liao
Jul 10, 2017·Bioorganic & Medicinal Chemistry Letters·Le Thi VienChau Van Minh
Citations
Jan 30, 2019·Phytotherapy Research : PTR·Min LiJunxing Dong
Feb 15, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mohamad Shazeli Che ZainKhozirah Shaari
Oct 20, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhenzhong YangXiaoping Zhao
May 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xianying FangFuliang Cao
Dec 5, 2019·Plants·Amélia M SilvaIsabel Gaivão
Dec 17, 2020·Journal of Food Biochemistry·Zhipeng QiLinguo Zhao
Dec 15, 2020·The American Journal of Chinese Medicine·Da-Sol KimHye-Ock Jang
Feb 3, 2021·Analytical Methods : Advancing Methods and Applications·Yun SunMenghui Cao
Apr 24, 2021·PeerJ·Shuheng ZhaoHong Chen
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fenfen WangShihua Shen
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ruihong LiJunxing Dong
Jul 30, 2021·Journal of Hazardous Materials·Yawen WuYijian Rao