Enrichment of benzo[a]pyrene in smoked food products and determination by high-performance liquid chromatography-fluorescence detection

Journal of Chromatography. a
M S García FalcónJ Simal Lozano

Abstract

We developed a procedure for trace enrichment of benzo[a]pyrene (BP) in extracts of smoked food products, and an HPLC-fluorescence detection (FL) method for determination of BP in the enriched extracts. The procedure consists in extraction/sonication of the lyophilized product in hexane, clean-up of the hexane extract by passage through a Sep-Pak Silica Plus cartridge and, subsequently, by partitioning between hexane and dimethyl sulphoxide, and concentration of the BP using a Sep-Pak C18 Plus cartridge. HPLC-FL and quantification limits were 0.049 microgram/l in acetonitrile (< 0.0067 microgram/kg of smoked food) and 0.089 microgram/l in acetonitrile (< 0.012 microgram/kg), respectively. Recovery (94.1%) and RSD (< 8.65%) were satisfactory. When applied to 15 types of sausage, mean BP content was 0.022 microgram/kg, and all but two samples (both treated with wood smoke) had BP contents below the 0.03 microgram/kg limit imposed in EU legislation for smoking-flavour agents.

References

Feb 1, 1978·Bulletin of Environmental Contamination and Toxicology·H BravoE F Mandelli
May 1, 1991·Food Additives and Contaminants·E MenichiniF Monfredini
Dec 1, 1990·Zeitschrift Für Lebensmittel-Untersuchung Und -Forschung·K SpeerA Montag
Dec 1, 1988·Zeitschrift Für Lebensmittel-Untersuchung Und -Forschung·B K LarssonM Sauri
Sep 30, 1988·Journal of Chromatography·D A BirkholzS E Hrudey
Aug 1, 1994·Bulletin of Environmental Contamination and Toxicology·V AkpanP Dolara
Sep 1, 1993·Food Additives and Contaminants·E A GomaaA M Booren
May 1, 1993·Archives of Environmental Contamination and Toxicology·H SetoY Kawahara

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Citations

Mar 18, 2004·Water Research·M S García-FalcónJ Simal-Gándara
Oct 31, 2008·Talanta·S Vazquez TrocheJ Simal Lozano
Oct 12, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Claude ViauAdolf Vyskocil
Sep 30, 2005·Food Additives and Contaminants·M S García-Falcón, J Simal-Gándara
Sep 22, 2001·Food Additives and Contaminants·M S García FalcónJ Simal Lozano
Dec 25, 2010·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·N LiY Q Li
Apr 26, 2014·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Euloge Yénoukounmè KpoclouMarie-Louise Scippo
Nov 8, 2005·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Maria Asunción Lage-YustyJesús Simal-Lozano
Sep 17, 2008·The Science of the Total Environment·Ledicia Rey-SalgueiroJesús Simal-Gándara
Feb 2, 2012·Toxicology and Industrial Health·Taha A KumosaniAmer Hamzah Asseri
Feb 17, 2005·Journal of Agricultural and Food Chemistry·Justin Koffi HouessouValérie Camel
Nov 13, 2004·Journal of Agricultural and Food Chemistry·M S García-FalcónJ Simal-Gándara

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