Enrichment of collagen plugs with platelets and amniotic fluid cells increases cell proliferation in sealed iatrogenic membrane defects in the foetal rabbit model

Prenatal Diagnosis
Daisy LiekensJ Deprest

Abstract

The purpose of this study was to evaluate cell proliferation in platelet-enriched collagen plugs with and without addition of amniotic fluid-derived heterologous foetal cells to seal an iatrogenic membrane defect in the foetal rabbit model. Amniotic fluid cells were harvested from three donor does at 23 days of gestation (term = 32 days) and labelled with carboxyfluorescein diacetate succinimidyl ester (CFDA-SE). In 42 other does, foetal membrane defects were induced by foetoscopic needle puncture at 23 days of gestation, and closed with either a platelet-enriched collagen plug with (n = 44) or without (n = 32) amniotic fluid cells. At 30 days of gestation, the defects were harvested and assessed microscopically. The plugs enriched with heterologous amniotic fluid cells more commonly had proliferating cells in the centre of the plug than those without cell addition. CFDA-SE labelling confirmed the presence of heterologous amniotic fluid cells over the entire membrane plug. Cell typing showed a mixture of fibroblasts and epithelial cells at the wound edges, whereas in the centre, there was an abundance of fibroblasts. When sealing iatrogenic membrane defects in the foetal rabbit model, enrichment of collagen plugs with platelets...Continue Reading

References

Aug 15, 1998·American Journal of Obstetrics and Gynecology·R GomezS M Berry
May 18, 1999·American Journal of Obstetrics and Gynecology·E GratacósJ A Deprest
Oct 16, 1999·American Journal of Obstetrics and Gynecology·F I LuksB van Der Wildt
Jan 29, 2000·American Journal of Obstetrics and Gynecology·E GratacósJ A Deprest
Sep 15, 2000·European Journal of Obstetrics, Gynecology, and Reproductive Biology·R DevliegerJ A Deprest
Aug 6, 2003·Blood·Pieternella S In 't AnkerHumphrey H H Kanhai
Apr 16, 2004·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Robert E Marx
Dec 8, 2004·Best Practice & Research. Clinical Obstetrics & Gynaecology·Dario Fauza
Jun 10, 2005·Acta Biochimica Et Biophysica Sinica·Xiao-Qi WangYan Tan
May 2, 2006·Stem Cells and Development·Shaun M KunisakiDario O Fauza
May 10, 2006·American Journal of Obstetrics and Gynecology·R DevliegerJ A Deprest
Sep 14, 2006·Fetal Diagnosis and Therapy·Nikolaos A PapadopulosEdgar Biemer
Mar 10, 2007·American Journal of Obstetrics and Gynecology·Nicole Ochsenbein-KölbleJan Deprest
Nov 6, 2007·Obstetrics and Gynecology·Ajit S MallikNikolaos A Papadopulos

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Citations

Oct 25, 2012·Stem Cells International·Kerry RennieMahmud Bani-Yaghoub
Jun 1, 2011·Expert Opinion on Biological Therapy·Petra A B KlemmtBernd Groner
May 21, 2011·Gynécologie, obstétrique & fertilité·J DeprestL Lewi
Nov 17, 2009·Seminars in Fetal & Neonatal Medicine·Jan A DeprestLiesbeth Lewi
Jun 10, 2011·Prenatal Diagnosis·Jan DeprestLiesbeth Lewi
Jun 27, 2013·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·A C EngelsJ A Deprest
Jan 26, 2010·American Journal of Obstetrics and Gynecology·Grozdana BilicAndreas H Zisch
Oct 23, 2015·Journal of Biomaterials Applications·Sabiniano RomanSheila MacNeil
Aug 14, 2020·Seminars in Immunopathology·Ignacio ValenzuelaLiesbeth Lewi

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