Enrichment of committed human nucleus pulposus cells expressing chondroitin sulfate proteoglycans under alginate encapsulation

Osteoarthritis and Cartilage
Yuan-Hui SunVictor Y L Leung

Abstract

Intervertebral disc (IVD) degeneration is associated with a malfunction of the nucleus pulposus (NP). Alginate culturing provides a favorable microenvironment for the phenotypic maintenance of chondrocyte-like NP cells. However, NP cells are recently evidenced to present heterogeneous populations, including progenitors, fibroblastic cells and primitive NP cells. The aim of this study is to profile the phenotypic changes of distinct human NP cells populations and describe the dynamic expression of chondroitin sulfate glycosaminoglycans (CS-GAGs) in extended alginate encapsulation. Non-degenerated (ND-NPC) and degenerated (D-NPC) NP cells were expanded in monolayers, and subject to 28-day culture in alginate after serial passaging. CS-GAG compositional expression in monolayer-/alginate-cultured NP cells was evaluated by carbohydrate electrophoresis. Cellular phenotypic changes were assessed by immunologic detection and gene expression analysis. Relative to D-NPC, ND-NPC displayed remarkably higher expression levels of chondroitin-4-sulfate GAGs over the 28-day culture. Compared with monolayer culture, ND-NPC showed increased NP marker expression of KRT18, KRT19, and CDH2, as well as chondrocyte markers SOX9 and MIA in alginate cu...Continue Reading

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Citations

Mar 29, 2018·Annals of Biomedical Engineering·Eric Y SunLeo Q Wan
Aug 24, 2018·Journal of Orthopaedic Surgery and Research·Ann-Kathrin SchubertJochen Ringe
Dec 19, 2020·Osteoarthritis and Cartilage·Jingcheng WangYueming Song
Aug 7, 2021·International Journal of Biological Macromolecules·Özge Karabıyık AcarGamze Torun Kose

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