PMID: 6168702Jan 1, 1981Paper

Enrichment of human basophils

Journal of Immunological Methods
J J Pruzansky, R Patterson

Abstract

A 3-step procedure is described in detail for producing 50% basophils in 50% yield from human blood. Leukocytes from dextran sedimented blood or preferably whole blood are sedimented on Hypaque-Ficoll, then on aqueous Hypaque, followed by adherence and elution from a column of glass beads. The cells appear normal and undergo specific histamine release after incubation with antigen. The procedure is relatively rapid and should allow further study of mechanisms of mediator release with more homogeneous populations of basophils. The first step also provides a 5 min separation of blood into a lymphocyte enriched mononuclear cell fraction and a granulocyte enriched fraction.

References

Jul 1, 1961·The Journal of Experimental Medicine·J E GARVIN

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Citations

Dec 4, 1987·Journal of Immunological Methods·J A WarnerD W MacGlashan
Feb 24, 1989·Journal of Immunological Methods·M A Lett-BrownJ A Grant
Oct 29, 1990·Journal of Immunological Methods·J T Schroeder, L R Hanrahan
Jan 4, 1993·Journal of Immunological Methods·T BjerkeP O Schiøtz
Nov 1, 1992·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Y TanimotoI Kimura
Sep 1, 1988·The Journal of Allergy and Clinical Immunology·H J DegenhartH J Neijens
Jan 1, 1990·Critical Reviews in Oncology/hematology·P Valent, P Bettelheim
Oct 28, 1983·Journal of Immunological Methods·F J Landry, S R Findlay
Apr 25, 2008·Current Protocols in Immunology·D D Metcalfe
Jan 1, 1983·Clinical and Laboratory Haematology·E B MitchellA D Webster

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