PMID: 7008901Mar 2, 1981Paper

Enrichment of Schwann cell cultures from neonatal rat sciatic nerve by differential adhesion

Brain Research
B KreiderD E Pleasure

Abstract

A novel method of Schwann cell purification from neonatal rat sciatic nerve has been developed using differential adhesion. After enzymatic and mechanical dissociation, the cell digest is allowed to settle on polylysine-coated glass coverslips for 30 min with intermittent shaking. After an 18-h incubation, bipolar cells comprise greater than 95% of the non-adherent population. Indirect immunofluorescence with the cell-specific markers rabbit anti-galactocerebroside and rabbit anti-bovine-P-2 basic protein antiserum confirmed light microscopic identification of these bipolar cells as Schwann cells. Rabbit anti-human fibronectin specifically labeled fibroblasts which comprised less than 5% of the cell population, but did not bind to Schwann cells. Schwann cells isolated by differential adhesion were injected into a rabbit. When absorbed with cultured rat skin fibroblasts, serum from this rabbit specifically surface labeled greater than 99% of the bipolar and round cells after 18 h and 5 days in vitro and also labeled Schwann cells in fetal rat dorsal root ganglia cultures, but not fibroblasts or neurons.

References

Jan 1, 1979·Journal of the Neurological Sciences·R P LisakD H Silberberg
Mar 24, 1977·Nature·J P BrockesM C Raff
May 16, 1975·Brain Research·R C WigginsP Morell

❮ Previous
Next ❯

Citations

Feb 1, 1992·Journal of Neuroscience Research·J KamholzD Pleasure
Feb 1, 1992·Journal of Neuroscience Research·M HardyD Pleasure
Apr 1, 1992·Journal of Neuroscience Research·P W MasonG H DeVries
Oct 1, 1992·Journal of Neuroscience Research·L M BolinE M Shooter
Jan 1, 1995·Journal of Neuroscience Research·B Q KreiderD Pleasure
Aug 1, 1995·Journal of Neuroscience Research·B T ZhangT Takenaka
Jan 1, 1984·Acta Neuropathologica·G MorettoN Rizzuro
Sep 1, 1983·Italian Journal of Neurological Sciences·R P LisakA J Summer
Dec 29, 2012·Purinergic Signalling·Tania MartiáñezAlejandro Gella
Nov 12, 1986·Brain Research·B Q KreiderF A McMorris
Dec 6, 1988·Brain Research·G SobueT Mitsuma
Feb 9, 1988·Brain Research·E ScarpiniG Scarlato
Jun 13, 1988·Journal of Immunological Methods·P J ArmatiL Van der Lubbe
Jun 1, 1989·Journal of Neuroscience Methods·Y OdaI Nakanishi
Sep 19, 1991·Brain Research. Developmental Brain Research·A Komiyama, K Suzuki
Jun 12, 1985·Neuroscience Letters·R P LisakD E Pleasure
May 22, 1989·Neuroscience Letters·T YasudaA Takahashi
Oct 1, 1993·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·G RaismanG A Brook
Apr 15, 1996·Toxicology·M KamijimaY Takeuchi
Apr 1, 1982·Journal of Neuroimmunology·K L Fields, C S Raine
Jan 1, 1982·Journal of Neuroimmunology·P G Kennedy
Jan 7, 2009·Journal of Neuroscience Research·Ivo Spiegel, Elior Peles
Apr 30, 1984·Biochemical and Biophysical Research Communications·S SegalD Pleasure

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.