Ensilication Improves the Thermal Stability of the Tuberculosis Antigen Ag85b and an Sbi-Ag85b Vaccine Conjugate

Scientific Reports
A A WahidJ M H van den Elsen

Abstract

There is an urgent need for the development of vaccine thermostabilisation methodologies as the maintenance of a continuous and reliable cold chain remains a major hurdle to the global distribution of safe and effective vaccines. Ensilication, a method that encases proteins in a resistant silica cage has been shown to physically prevent the thermal denaturation of a number of model proteins. In this study we investigate the utility of this promising approach in improving the thermal stability of antigens and vaccine conjugates highly relevant to the development of candidate tuberculosis vaccines, including antigen 85b conjugated with the Staphylococcus aureus-protein based adjuvant Sbi. Here we analyse the sensitivity of these constructs to thermal denaturation and demonstrate for the first time the benefits of ensilication in conferring these vaccine-relevant proteins with protection against temperature-induced loss of structure and function without the need for refrigeration. Our results reveal the potential of ensilication in facilitating the storage and transport of vaccines at ambient temperatures in the future and therefore in delivering life-saving vaccines globally, and in particular to remote areas of developing countr...Continue Reading

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Citations

Aug 31, 2020·Clinical and Experimental Vaccine Research·Bonnie Leigh Russell, Samantha Gildenhuys
Dec 20, 2020·Advanced Drug Delivery Reviews·Kyung Soo ParkJames J Moon
Mar 17, 2021·Expert Review of Vaccines·Yizhi Qi, Christopher B Fox
May 11, 2021·Veterinary Immunology and Immunopathology·Kezia R FourieHeather L Wilson
Oct 24, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Nicole A MontoyaMark B Shiflett
Oct 5, 2021·Advanced Healthcare Materials·Fernanda LangellottoDavid J Mooney

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Methods Mentioned

BETA
circular dichroism
size exclusion chromatography
dynamic light scattering
ion exchange chromatography
scanning electron microscopy

Software Mentioned

UCSF Chimera
GraphPad Prism
DichroWeb
OriginLab

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