Entactin: a possible auto-antigen in the pathogenesis of non-Goodpasture anti-GBM nephritis

Kidney International
R SaxenaJ Wieslander

Abstract

It has recently been demonstrated that many patients with various types of glomerulonephritis have antibodies to the 6M guanidine-HCl extract of glomerular basement membrane (Bygren et al, Nephrol Dial Transplant 4:254-261, 1989). In the present study a 150 K protein was isolated from the guanidine extract of bovine glomerular basement membrane utilizing ion exchange and gel filtration chromatographic procedures. Amino acid analysis and size of the isolated protein revealed similarity to that of entactin/nidogen. The identity of this protein as entactin/nidogen was further suggested by its precipitation with two different antibodies in a radioimmunoassay and by its reaction with four different antibodies in a sandwich ELISA. Inhibition of the antibodies to 150 K by bovine entactin, which was isolated separately and sequenced for amino acids, confirmed the identity of the 150 K protein as entactin/nidogen. Furthermore, it was shown that about one third of those patients who show antibodies to the crude guanidine extract have circulating antibodies directed against entactin. This was further confirmed by the competitive inhibition of antibodies to the crude guanidine extract in one of the positive serum by entactin in an ELISA in...Continue Reading

References

Sep 1, 1978·The American Journal of Medicine·P A MorrinH Kreis
Jan 1, 1989·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·P BygrenJ Wieslander
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B CederholmD Heinegård
Jun 1, 1974·Kidney International·C B Wilson, F J Dixon
Feb 1, 1973·Kidney International·C B Wilson, F J Dixon
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J WieslanderD Heinegård
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J WieslanderB G Hudson
Jan 1, 1984·Infection and Immunity·J L AvilaM Rieber
Dec 15, 1983·European Journal of Biochemistry·R TimplG Wick
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D Kerjaschki, M G Farquhar
Apr 1, 1982·The Journal of Experimental Medicine·A SzarfmanG R Martin

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Citations

Sep 19, 2003·European Journal of Internal Medicine·A RutgersJ W. Tervaert
Apr 1, 1992·Postgraduate Medical Journal·R SaxenaJ Wieslander
Feb 1, 1995·European Journal of Biochemistry·U MayerR Nischt
Mar 1, 1992·Kidney International·M Weber
Oct 1, 1991·Kidney International·A KatzR J Butkowski
May 1, 1991·Kidney International·R SaxenaJ Wieslander
Oct 30, 2016·The American Journal of Pathology·Ole P RekvigNatalya Seredkina
Jul 14, 2006·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Sevcan Azime BakkalogluNecla Buyan
Oct 14, 2018·Journal of the American Society of Nephrology : JASN·Stephen P McAdoo, Charles D Pusey

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