Entamoeba histolytica Induce Signaling via Raf/MEK/ERK for Neutrophil Extracellular Trap (NET) Formation

Frontiers in Cellular and Infection Microbiology
Zayda FonsecaCarlos Rosales

Abstract

Amoebiasis, the disease caused by Entamoeba histolytica is the third leading cause of human deaths among parasite infections. E. histolytica was reported associated with around 100 million cases of amoebic dysentery, colitis and amoebic liver abscess that lead to almost 50,000 fatalities worldwide in 2010. E. histolytica infection is associated with the induction of inflammation characterized by a large number of infiltrating neutrophils. These neutrophils have been implicated in defense against this parasite, by mechanisms not completely described. The neutrophil antimicrobial mechanisms include phagocytosis, degranulation, and formation of neutrophil extracellular traps (NETs). Recently, our group reported that NETs are also produced in response to E. histolytica trophozoites. But, the mechanism for NETs induction remains unknown. In this report we explored the possibility that E. histolytica leads to NETs formation via a signaling pathway similar to the pathways activated by PMA or the Fc receptor FcγRIIIb. Neutrophils were stimulated by E. histolytica trophozoites and the effect of various pharmacological inhibitors on amoeba-induced NETs formation was assessed. Selective inhibitors of Raf, MEK, and NF-κB prevented E. histo...Continue Reading

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Citations

Dec 1, 2018·Bioscience Reports·César Díaz-Godínez, Julio C Carrero
Jun 4, 2020·Physiology·Eileen Uribe-Querol, Carlos Rosales
Oct 8, 2019·International Journal of Medical Microbiology : IJMM·Julio C CarreroMireya de la Garza
Dec 23, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Di WuZhengkai Wei
Nov 18, 2020·Biochemistry. Biokhimii︠a︡·N V Vorobjeva, B V Chernyak
Sep 4, 2021·Frontiers in Oncology·Bo-Zong ShaoEn-Qiang Linghu

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Methods Mentioned

BETA
PMA
flow cytometry
fluorescence spectroscopy
fluorescence microscopy
flow
GTPase
GTPases

Software Mentioned

EU
Q Capture pro
Fiji
ImageJ
KaleidaGraph®version
Elmer FL WinLab
Cellquest
Perkin

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