Entamoeba invadens: Identification of a SERCA protein and effect of SERCA inhibitors on encystation

Microbial Pathogenesis
Aarón Martinez-HigueraMario A Rodríguez

Abstract

Calcium has an important role on signaling of different cellular processes, including growth and differentiation. Signaling by calcium also has an essential function in pathogenesis and differentiation of the protozoan parasites Entamoeba histolytica and Entamoeba invadens. However, the proteins of these parasites that regulate the cytoplasmic concentration of this ion are poorly studied. In eukaryotic cells, the calcium-ATPase of the SERCA type plays an important role in calcium homeostasis by catalyzing the active efflux of calcium from cytoplasm to endoplasmic reticulum. Here, we reported the identification of SERCA of E. invadens (EiSERCA). This protein contains a putative sequence for endoplasmic reticulum retention and all domains involved in calcium transport identified in mammalian SERCA. By immunofluorescence assays, an antibody against SERCA of E. histolytica detected EiSERCA in a vesicular network in the cytoplasm of E. invadens trophozoites, co-localizing with calreticulin. Interestingly, EiSERCA was redistributed close to plasma membrane during encystation, suggesting that this pump could participate in regulate the calcium concentration during this process. In addition, thapsigargin and cyclopiazonic acid, both sp...Continue Reading

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Citations

Oct 18, 2018·Frontiers in Cellular and Infection Microbiology·Martha Valle-SolisMario A Rodríguez
May 2, 2020·Chemical Biology & Drug Design·Aadish RawatVijay Kumar Srivastava
Apr 11, 2018·Frontiers in Chemistry·Anna MeierEric Beitz

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