Entamoeba shows reversible variation in ploidy under different growth conditions and between life cycle phases.

PLoS Neglected Tropical Diseases
Chandrama MukherjeeAnuradha Lohia

Abstract

Under axenic growth conditions, trophozoites of Entamoeba histolytica contain heterogenous amounts of DNA due to the presence of both multiple nuclei and different amounts of DNA in individual nuclei. In order to establish if the DNA content and the observed heterogeneity is maintained during different growth conditions, we have compared E. histolytica cells growing in xenic and axenic cultures. Our results show that the nuclear DNA content of E. histolytica trophozoites growing in axenic cultures is at least 10 fold higher than in xenic cultures. Re-association of axenic cultures with their bacterial flora led to a reduction of DNA content to the original xenic values. Thus switching between xenic and axenic growth conditions was accompanied by significant changes in the nuclear DNA content of this parasite. Changes in DNA content during encystation-excystation were studied in the related reptilian parasite E. invadens. During excystation of E. invadens cysts, it was observed that the nuclear DNA content increased approximately 40 fold following emergence of trophozoites in axenic cultures. Based on the observed large changes in nuclear size and DNA content, and the minor differences in relative abundance of representative pro...Continue Reading

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Citations

Feb 20, 2010·PLoS Neglected Tropical Diseases·Hugo Aguilar-DíazJulio C Carrero
May 29, 2012·Genome Biology·Gareth D WeedallNeil Hall
Dec 17, 2014·Computational and Structural Biotechnology Journal·Koushik Das, Sandipan Ganguly
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May 1, 2021·Microorganisms·Jordan WeselCheryl Ingram-Smith
Jun 23, 2021·Acta Parasitologica·Marianna de Carvalho ClímacoSilvio Santana Dolabella

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Methods Mentioned

BETA
flow cytometry
PCR

Software Mentioned

Kyplot
CELLQUEST
Metafer

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Amoebiasis, infection by the protozoan parasite Entamoeba histolytica, remains a global health problem, despite the availability of effective treatment. Here is the latest research.

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