Enteric and nasal shedding of bovine torovirus (Breda virus) in feedlot cattle

American Journal of Veterinary Research
A E HoetL J Saif

Abstract

To assess fecal and nasal shedding patterns of bovine torovirus (BoTV) in cattle at time of arrival and periodically throughout the first 21 days after arrival at a feedlot. 57 steers. Fecal and nasal-swab samples collected on days 0, 4, 14, and 21 after arrival were tested for BoTV, using ELISA. A subset of samples from calves testing positive and negative for BoTV was analyzed, using reverse transcriptase-polymerase chain reaction (RT-PCR). Paired serum samples were collected on days 0 and 21 and tested for BoTV antibodies, using a hemagglutination inhibition assay. Overall rate of fecal shedding of BoTV was 21 of 57 (37%) by ELISA and 40 of 42 (95%) by RT-PCR with peak shedding on day 4. Diarrhea was more common in calves shedding BoTV than those not shedding the virus (odds ratio, 1.72). Overall rate of nasal shedding of BoTV was 15 of 57 (26%) by ELISA and 42 of 42 (100%) by RT-PCR, with peak shedding on day 0. Specificity of the RT-PCR product was confirmed by sequence analysis. Approximately 93% of the calves seroconverted to BoTV (> 4-fold increase in titer). Differences were not detected between calves shedding BoTV and nonshedders in relation to disease and treatments, perhaps because of the low number of cattle in th...Continue Reading

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Citations

Dec 15, 2011·Archives of Virology·Tsunehiko AitaHiroshi Tsunemitsu
Jun 30, 2005·Animal Health Research Reviews·Armando E Hoet, Linda J Saif
Apr 16, 2003·American Journal of Veterinary Research·Armando E HoetLinda J Saif
Jan 15, 2014·Archives of Virology·Irem GülaçtıIbrahim Sözdutmaz
Dec 29, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Mika ItoMakoto Nagai
Jan 9, 2009·Veterinary Microbiology·Toshihiro ItoMitsugu Shimizu
Aug 28, 2007·Veterinary Microbiology·Su-Jin ParkKyoung-Oh Cho
Aug 3, 2007·Veterinary Microbiology·Nicola DecaroCanio Buonavoglia
Feb 27, 2007·Virus Research·Toshihiro ItoShin-ichi Fukuyama
Apr 25, 2006·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·B HaschekW Baumgartner
May 9, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Armando E HoetLinda J Saif
Jan 1, 2014·Journal of Veterinary Science·Yong-Il Cho, Kyoung-Jin Yoon
Jan 14, 2020·Tropical Animal Health and Production·Ji-Hyoung RyuKyoung-Seong Choi
Jun 15, 2007·Clinical and Vaccine Immunology : CVI·Masaki KuwabaraHiroshi Tsunemitsu
Jul 10, 2020·Journal of Virology·Eva DervasAnja Kipar

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