Entering an era of dynamic structural biology…

BMC Biology
Allen M Orville

Abstract

A recent paper in BMC Biology presents a general method for mix-and-inject serial crystallography, to facilitate the visualization of enzyme intermediates via time-resolved serial femtosecond crystallography (tr-SFX). They apply their method to resolve in near atomic detail the cleavage and inactivation of the antibiotic ceftriaxone by a β-lactamase enzyme from Mycobacterium tuberculosis. Their work demonstrates the general applicability of time-resolved crystallography, from which dynamic structures, at atomic resolution, can be obtained.See research article: https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-018-0524-5 .

References

Mar 15, 2006·Structure·Silvano GeremiaLouise N Johnson
Jul 25, 2017·Trends in Biochemical Sciences·Linda C JohanssonVadim Cherezov
Nov 4, 2017·Nature Communications·A MeentsH N Chapman
Mar 14, 2018·Acta Crystallographica. Section D, Structural Biology·Jonathan M GrimesDavid I Stuart

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Citations

Jun 21, 2019·PLoS Computational Biology·Ruth NussinovHyunbum Jang
Oct 15, 2020·Communications Biology·Qing-di ChengChristian Betzel
Sep 21, 2020·Journal of Structural Biology·Nate YoderEric Gouaux
Jul 24, 2021·Nature Communications·Agata ButrynAllen M Orville

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Methods Mentioned

BETA
X-ray

Software Mentioned

VMXi

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