Enterobacter cloacae administration induces hepatic damage and subcutaneous fat accumulation in high-fat diet fed mice

PloS One
Anniina KeskitaloSatu Pekkala

Abstract

Accumulating evidence indicates that gut microbiota plays a significant role in obesity, insulin resistance and associated liver disorders. Family Enterobacteriaceae and especially Enterobacter cloacae strain B29 have been previously linked to obesity and hepatic damage. The underlying mechanisms, however, remain unclear. Therefore, we comprehensively examined the effects of E. cloacae subsp. cloacae (ATCC® 13047™) administration on host metabolism of mice fed with high-fat diet (HFD). C57BL/6N mice were randomly divided into HFD control, chow control, and E. cloacae treatment groups. The E. cloacae treatment group received live bacterial cells in PBS intragastrically twice a week, every other week for 13 weeks. Both control groups received PBS intragastrically. After the 13-week treatment period, the mice were sacrificed for gene and protein expression and functional analyses. Our results show that E. cloacae administration increased subcutaneous fat mass and the relative proportion of hypertrophic adipocytes. Both subcutaneous and visceral fat had signs of decreased insulin signaling and elevated lipolysis that was reflected in higher serum glycerol levels. In addition, E. cloacae -treated mice had significantly higher hepati...Continue Reading

References

Jan 1, 1990·Reviews of Infectious Diseases·A A LindbergE T Rietschel
Apr 1, 1997·Clinical Microbiology Reviews·W E Sanders, C C Sanders
Sep 13, 2002·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S-O LeeJ Ryu
Oct 16, 2004·Science·Umut OzcanGökhan S Hotamisligil
Oct 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Fredrik BäckhedJeffrey I Gordon
Aug 17, 2006·Liver International : Official Journal of the International Association for the Study of the Liver·Helena NyblomRolf Olsson
Oct 7, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Paola BrunDiego Martines
Dec 22, 2006·Nature·Ruth E LeyJeffrey I Gordon
Apr 26, 2007·Diabetes·Patrice D CaniRémy Burcelin
Feb 28, 2009·Cell Death and Differentiation·Y LiuY Shinomura
Mar 18, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Luca MieleAntonio Grieco
Nov 11, 2009·Current Opinion in Gastroenterology·Ruth E Ley
Feb 13, 2010·Annual Review of Physiology·Jerrold M Olefsky, Christopher K Glass
Jan 14, 2011·Annual Review of Medicine·Giovanni MussoMaurizio Cassader
May 17, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yanfei ChenLanjuan Li
Sep 29, 2011·Diabetes·Fredrik KarpeKeith N Frayn
May 2, 2012·Obesity·Caroline L J KarlssonKristina Thorngren-Jerneck
Aug 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Qingqing LiuHarvey F Lodish
Sep 14, 2012·Nature·Catherine A LozuponeRob Knight
Sep 14, 2012·Nature·Valentina Tremaroli, Fredrik Bäckhed
Oct 12, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Lixin ZhuSteven R Gill
Apr 26, 2013·Briefings in Functional Genomics·Patrice D Cani
Jul 10, 2013·Journal of Lipid Research·Antonio GiordanoSaverio Cinti
Aug 27, 2013·Immunity·Leonid YurkovetskiyAlexander V Chervonsky
Jan 1, 2014·Nature Reviews. Drug Discovery·Robert SabatKerstin Wolk
Mar 1, 2014·Journal of Molecular Endocrinology·Thomas Svava NielsenSten Lund
Jun 17, 2014·Cell Metabolism·Ingrid Wernstedt AsterholmPhilipp E Scherer
Sep 23, 2014·Molecular Genetics and Metabolism·Chiara Caselli
Nov 8, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Philana FernandesAileen Houston
Jan 21, 2015·Matrix Biology : Journal of the International Society for Matrix Biology·Sergio DuarteAna J Coito
Mar 4, 2015·The Journal of Cell Biology·Joseph M RutkowskiPhilipp E Scherer
Mar 12, 2015·Journal of Clinical Biochemistry and Nutrition·Risa TairaToshio Taira
Mar 31, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sena KimHun Taeg Chung
Dec 3, 2015·American Journal of Physiology. Endocrinology and Metabolism·Stefan R HargettSusanna R Keller
Feb 13, 2016·Arteriosclerosis, Thrombosis, and Vascular Biology·Daniel B HarmonColeen A McNamara
May 27, 2016·PloS One·Carmen HaroAntonio Camargo

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Citations

Jan 1, 2018·Nuclear Receptor Research·Danika BakkeJun Sun
Oct 3, 2021·Diabetes Research and Clinical Practice·Patricia Medici DualibBianca de Almeida Pititto

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Methods Mentioned

BETA
PCR
SMA

Software Mentioned

SPSS Statistics
JMP Pro
[UNK]
CellProfiler

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