PMID: 25773833Mar 17, 2015Paper

Enterocarpam-III induces human liver and breast cancer cell apoptosis via mitochondrial and caspase-9 activation

Asian Pacific Journal of Cancer Prevention : APJCP
Ratana BanjerdpongchaiWilart Pompimon

Abstract

An aristolactam-type alkaloid, isolated from Orophea enterocarpa, is enterocarpam-III (10-amino-2,3,4,6- tetramethoxyphenanthrene-1-carboxylic acid lactam). It is cytotoxic to various human and murine cancer cell lines; however, the molecular mechanisms remain unclear. The aims of this study were to investigate cytotoxic effects on and mechanism (s) of human cancer cell death in human hepatocellular carcinoma HepG2 and human invasive breast cancer MDA-MB-231 cells compared to normal murine fibroblast NIH3T3 cells. Cell viability was determined by MTT assay to determine IC10, IC20 and IC50 levels, reactive oxygen species (ROS) production with 2',7'-dichlorohydrofluorescein diacetate and the caspase-3, -8 and -9 activities using specific chromogenic (p-nitroaniline) tetrapeptide substrates, viz., DEVD-NA, IETD-NA and LEHD-NA and employing a microplate reader. Mitochondrial transmembrane potential (MTP) was measured by staining with 3, 3'-dihexyloxacarbocyanine iodide (DiOC6) and using flow cytometry. The compound was cytotoxic to HepG2 and MDA-MB-231 cells with the IC50 levels of 26.0±4.45 and 51.3±2.05 μM, respectively. For murine normal fibroblast NIH3T3 cells, the IC50 concentration was 81.3±10.1 μM. ROS production was reduced...Continue Reading

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Jun 4, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Ratana BanjerdpongchaiWilart Pompimon
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Jan 1, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Ratana BanjerdpongchaiKorawan Sringarm

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Citations

Feb 15, 2021·Applied Microbiology and Biotechnology·Xiao WangDengke Yin

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