Enterococcus bulliens sp. nov., a novel lactic acid bacterium isolated from camel milk

Antonie van Leeuwenhoek
Zaina KadriPeter Vandamme

Abstract

Four lactic acid bacteria isolates obtained from fresh dromedary camel milk produced in Dakhla, a city in southern Morocco, were characterised in order to determine their taxonomic position. The four isolates had highly similar MALDI-TOF MS and RAPD fingerprints and identical 16S rRNA gene sequences. Comparative sequence analysis revealed that the 16S rRNA gene sequence of the four isolates was most similar to that of Enterococcus sulfureus ATCC 49903(T) and Enterococcus italicus DSM 15952(T) (99.33 and 98.59% similarity, respectively). However, sequence analysis of the phenylalanyl-tRNA synthase (pheS), RNA polymerase (rpoA) and ATP synthase (atpA) genes revealed that the taxon represented by strain LMG 28766(T) was well separated from E. sulfureus LMG 13084(T) and E. italicus LMG 22039(T), which was further confirmed by DNA-DNA hybridization values that were clearly below the species demarcation threshold. The novel taxon was easily differentiated from its nearest neighbour species through sequence analysis of protein encoding genes, MALDI-TOF mass spectrometry and multiple biochemical tests, but had a similar percentage G+C content of about 39%. We therefore propose to formally classify these isolates as Enterococcus bullien...Continue Reading

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Citations

Feb 13, 2016·International Journal of Systematic and Evolutionary Microbiology·Aharon Oren, George M Garrity
Jun 27, 2019·BioMed Research International·Olfa Ben Braïek, Slim Smaoui
Jan 12, 2020·Critical Reviews in Food Science and Nutrition·Ken GrahamRosemary Rea
Jan 27, 2021·International Journal of Food Microbiology·Zaina KadriPeter Vandamme
Feb 24, 2021·International Journal of Systematic and Evolutionary Microbiology·Yu Qin Li, Chun Tao Gu

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