Enterococcus faecalis acetoacetyl-coenzyme A thiolase/3-hydroxy-3-methylglutaryl-coenzyme A reductase, a dual-function protein of isopentenyl diphosphate biosynthesis.

Journal of Bacteriology
Matija HedlV W Rodwell

Abstract

Many bacteria employ the nonmevalonate pathway for synthesis of isopentenyl diphosphate, the monomer unit for isoprenoid biosynthesis. However, gram-positive cocci exclusively use the mevalonate pathway, which is essential for their growth (E. I. Wilding et al., J. Bacteriol. 182:4319-4327, 2000). Enzymes of the mevalonate pathway are thus potential targets for drug intervention. Uniquely, the enterococci possess a single open reading frame, mvaE, that appears to encode two enzymes of the mevalonate pathway, acetoacetyl-coenzyme A thiolase and 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase. Western blotting revealed that the mvaE gene product is a single polypeptide in Enterococcus faecalis, Enterococcus faecium, and Enterococcus hirae. The mvaE gene was cloned from E. faecalis and was expressed with an N-terminal His tag in Escherichia coli. The gene product was then purified by nickel affinity chromatography. As predicted, the 86.5-kDa mvaE gene product catalyzed both the acetoacetyl-CoA thiolase and HMG-CoA reductase reactions. Temperature optima, DeltaH(a) and K(m) values, and pH optima were determined for both activities. Kinetic studies of acetoacetyl-CoA thiolase implicated a ping-pong mechanism. CoA acted as ...Continue Reading

References

Jan 1, 1975·Methods in Enzymology·K D ClinkenbeardM D Lane
Apr 1, 1987·Archives of Biochemistry and Biophysics·F SuzukiD W Emerich
Jan 1, 1995·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·A CorsiniA L Catapano
Jun 1, 1997·Biochemical and Molecular Medicine·K S RogersP Geiger
Mar 9, 1999·Molecular Genetics and Metabolism·D A BocharV W Rodwell
Apr 15, 2000·Journal of Molecular Biology·Y Modis, R K Wierenga
Jul 13, 2000·Protein Science : a Publication of the Protein Society·D Y KimV W Rodwell
Nov 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·B M LangeR Croteau

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Citations

Mar 19, 2004·Journal of Bacteriology·Matija HedlVictor W Rodwell
May 24, 2006·Bioscience, Biotechnology, and Biochemistry·Hiroshi Yoneyama, Ryoichi Katsumata
Nov 16, 2006·PLoS Biology·Kathleen M ScottGary T Zeruth
Dec 10, 2013·Biochimica Et Biophysica Acta·Benjamin H SchwarzJeffrey M Watson
Feb 19, 2013·Plant Science : an International Journal of Experimental Plant Biology·Andréa Hemmerlin
Apr 2, 2005·Protein Science : a Publication of the Protein Society·Stephanie S DounVictor W Rodwell
May 21, 2004·Protein Science : a Publication of the Protein Society·Matija Hedl, Victor W Rodwell
Mar 14, 2014·Protein Science : a Publication of the Protein Society·Tyler P KormanJames U Bowie
Feb 11, 2014·Photosynthesis Research·Fiona K DaviesMatthew C Posewitz
Aug 26, 2014·PloS One·Sofia B Rodriguez, Thomas S Leyh
Aug 30, 2016·Journal of Bioscience and Bioengineering·Keisuke WadaHiroshi Shimizu
Aug 3, 2004·Biotechnology and Bioengineering·Autumn Sutherlin, Victor W Rodwell
May 7, 2011·Natural Product Reports·Tohru DairiIsao Fujii
Mar 7, 2003·The Journal of Biological Chemistry·Lydia TaberneroCynthia V Stauffacher
Aug 5, 2004·The Journal of Biological Chemistry·Nino CampobassoMichael N Gwynn
Dec 22, 2017·Applied Microbiology and Biotechnology·Yujin CaoHuizhou Liu
Jul 6, 2016·Acta Crystallographica. Section F, Structural Biology Communications·M IthayarajaM R N Murthy
Jan 15, 2019·Critical Reviews in Food Science and Nutrition·K Srinivasan, Elna M Buys
Mar 16, 2017·Current Opinion in Biotechnology·Chonglong WangSeon-Won Kim
Sep 4, 2020·Biotechnology Advances·Yuyao RenYongjin J Zhou
Aug 6, 2021·Journal of Industrial Microbiology & Biotechnology·Scott A WegnerJosé L Avalos

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