Enterococcus faecalis strains show culture heterogeneity in cell surface charge

Microbiology
Annet E J van MerodeBastiaan P Krom

Abstract

Adhesion of micro-organisms to biotic and abiotic surfaces is an important virulence factor and involves different types of interactions. Enterococcus faecalis, a human commensal and an important opportunistic pathogen, has the ability to adhere to surfaces. Biliary stents frequently become clogged with bacterial biofilms, with E. faecalis as one of the predominant species. Six E. faecalis strains isolated from clogged biliary stents were investigated for the presence of specific biochemical factors involved in their adhesion: aggregation substances (Aggs) and the enterococcal surface protein (encoded by the esp gene). In addition, physico-chemical factors involved in adhesion (zeta potential and cell surface hydrophobicity) were determined, as well as the influence of ox bile on these properties. Two-thirds of the biliary stent isolates displayed culture heterogeneity in the pH dependence of their zeta potentials. Moreover, 24 out of 46 clinical isolates of E. faecalis, including 11 laboratory strains, also displayed such heterogeneity. The culture heterogeneity was demonstrated to be a stable trait, not caused by quorum sensing, not plasmid mediated, and independent of the presence of esp and Agg. Data presented show that cul...Continue Reading

References

Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·G M DunnyD B Clewell
Nov 1, 1992·Journal of Dental Research·M M CowanH J Busscher
Oct 1, 1991·Antonie van Leeuwenhoek·D B KellH V Westerhoff
Nov 1, 1991·Scandinavian Journal of Gastroenterology·N DowidarP Matzen
Aug 1, 1987·Applied and Environmental Microbiology·M C van LoosdrechtA J Zehnder
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·Y IkeD B Clewell
Nov 1, 1993·Bio/technology·R C Ebersole, R M McCormick
Feb 7, 2001·Applied and Environmental Microbiology·H C van der Mei, H J Busscher
Apr 26, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D E LowR N Jones
Sep 26, 2001·Applied and Environmental Microbiology·A Toledo-AranaI Lasa
Oct 31, 2002·Applied and Environmental Microbiology·Sheryl H StregerPaul B Hatzinger
Feb 11, 2003·Microbiology·Leendert W HamoenOscar P Kuipers
Aug 7, 2004·Journal of Clinical Microbiology·Gianfranco DonelliBruna Facinelli
Sep 24, 2004·Infection and Immunity·Preeti M TendolkarNathan Shankar
Oct 8, 2004·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Güven Kayaoglu, Dag Ørstavik

❮ Previous
Next ❯

Citations

Jan 24, 2012·Applied and Environmental Microbiology·Muhammad TariqBastiaan P Krom
Sep 7, 2012·PLoS Pathogens·Henny C van der Mei, Henk J Busscher
Dec 25, 2010·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Albert BolhuisJanice Aldrich-Wright
Mar 25, 2008·Colloids and Surfaces. B, Biointerfaces·Annet E J van MerodeBastiaan P Krom
Apr 24, 2007·Journal of Applied Microbiology·A E J van MerodeB P Krom
Nov 19, 2011·Journal of Applied Microbiology·T JacquetA-M Revol-Junelles
Jan 31, 2018·Scientific Reports·Natalia NunezCristel Archambaud
Aug 29, 2020·AAPS PharmSciTech·Pedro Henrique Sette-de-SouzaAna Cláudia Dantas de Medeiros

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.