PMID: 9546189Apr 18, 1998Paper

Enterohemorrhagic Escherichia coli O157:H7 present in radish sprouts

Applied and Environmental Microbiology
Y ItohS Kumagai

Abstract

Using cultivation, immunofluorescence microscopy, and scanning electron microscopy, we demonstrated the presence of viable enterohemorrhagic Escherichia coli O157:H7 not only on the outer surfaces but also in the inner tissues and stomata of cotyledons of radish sprouts grown from seeds experimentally contaminated with the bacterium. HgCl2 treatment of the outer surface of the hypocotyl did not kill the contaminating bacteria, which emphasized the importance of either using seeds free from E. coli O157:H7 in the production of radish sprouts or heating the sprouts before they are eaten.

References

Feb 18, 1995·Lancet·A PönkäP Pakkala
Mar 3, 1997·International Journal of Food Microbiology·L R Beuchat
Sep 1, 1997·Journal of Food Protection·Y Hara-KudoS Kumagai

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Citations

Nov 20, 2001·Australian Veterinary Journal·G Hallaran, J Sumner
Jul 24, 2004·Journal of Food Protection·Lingeng LuSuresh D Pillai
Aug 30, 2008·Critical Reviews in Microbiology·Eelco Franz, Ariena H C van Bruggen
Jan 17, 2012·Annual Review of Food Science and Technology·Marilyn C Erickson
Jan 12, 2013·FEMS Microbiology Letters·Samuel Mohammed ChekababJosée Harel
Jul 6, 2000·Symposium Series·H Chart
Apr 20, 2001·Critical Reviews in Biotechnology·S ParkR A Durst
Sep 13, 2003·Journal of Applied Microbiology·K WarrinerW M Waites
Jul 31, 2014·Foodborne Pathogens and Disease·Amy M DechetBarbara E Mahon
Feb 24, 2016·Scientific Reports·Jae Hee Jung, Jung Eun Lee
May 29, 2000·Journal of Food Protection·W F Fett
Oct 8, 1999·Emerging Infectious Diseases·P J TaorminaL Slutsker
Jun 1, 2002·Applied and Environmental Microbiology·A O CharkowskiR E Mandrell
Oct 29, 2004·Journal of Food Protection·Sumathi SivapalasingamRobert V Tauxe
Nov 18, 2000·Journal of Food Protection·S J BuchkoD M Veira
Apr 16, 2005·Journal of Food Protection·Ethan B Solomon, Karl R Matthews
Jul 20, 2010·Environmental Microbiology·Cedric N BergerGad Frankel
Oct 24, 2003·Journal of Food Protection·Keith WarrinerWilliam M Waites
Apr 25, 2006·FEMS Microbiology Ecology·Stefan KutterMichael Schmid
Feb 27, 2015·Journal of Food Protection·Bismarck MartinezGlen Weaver
Apr 20, 2001·Journal of Food Protection·W R WeissingerL R Beuchat
Jun 29, 2002·Applied and Environmental Microbiology·Xuan GuoLarry R Beuchat
Apr 19, 2003·Emerging Infectious Diseases·Christopher J GillPaul R Cieslak

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