Enterohemorrhagic Escherichia coli senses low biotin status in the large intestine for colonization and infection

Nature Communications
Bin YangLei Wang

Abstract

Enterohemorrhagic Escherichia coli (EHEC) is an important foodborne pathogen that infects humans by colonizing the large intestine. Here we identify a virulence-regulating pathway in which the biotin protein ligase BirA signals to the global regulator Fur, which in turn activates LEE (locus of enterocyte effacement) genes to promote EHEC adherence in the low-biotin large intestine. LEE genes are repressed in the high-biotin small intestine, thus preventing adherence and ensuring selective colonization of the large intestine. The presence of this pathway in all nine EHEC serotypes tested indicates that it is an important evolutionary strategy for EHEC. The pathway is incomplete in closely related small-intestinal enteropathogenic E. coli due to the lack of the Fur response to BirA. Mice fed with a biotin-rich diet show significantly reduced EHEC adherence, indicating that biotin might be useful to prevent EHEC infection in humans.

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Citations

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Datasets Mentioned

BETA
SRR1619465
SRR1641752
SRR1621223
SRR1621224

Methods Mentioned

BETA
RNA-Seq
immunoprecipitation
PCR
ChIP-Seq
ChIP
electrophoresis

Software Mentioned

Solexa
FastQC
edgeR

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