Enteropathogenic Escherichia coli infection leads to appearance of aberrant tight junctions strands in the lateral membrane of intestinal epithelial cells

Cellular Microbiology
Michelle M Muza-MoonsGail Hecht

Abstract

Infection of intestinal epithelial cells with enteropathogenic Escherichia coli (EPEC) disrupts tight junction (TJ) architecture and barrier function. The aim of this study was to determine the impact of EPEC on TJ protein interactions and localization. Human intestinal epithelial cells (T84) were infected for 1, 3 or 6 h with EPEC. To probe the TJ protein-protein interactions, co-immunoprecipitations were performed. The associations between ZO-1, occludin and claudin-1 progressively decreased after infection. Corresponding morphological changes were analysed by immunofluorescence confocal microscopy. Tight junction proteins progressively lost their apically restricted localization. Freeze-fracture electron microscopy revealed the appearance of aberrant strands throughout the lateral membrane that contained claudin-1 and occludin as determined by immunogold labelling. These structural alterations were accompanied by a loss of barrier function. Mutation of the gene encoding EspF, important in the disruption of TJs by EPEC, prevented the disruption of TJs. Tight junction structure normalized following eradication of EPEC with gentamicin and overnight recovery. This is the first demonstration that a microbial pathogen can cause ab...Continue Reading

References

Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·T K McDanielJ B Kaper
Mar 1, 1995·The Journal of Clinical Investigation·I JustK Aktories
Apr 8, 1994·The Journal of Biological Chemistry·I JustC von Eichel-Streiber
Dec 1, 1993·The Journal of Cell Biology·B M Gumbiner
Dec 1, 1993·The Journal of Cell Biology·M FuruseS Tsukita
Jun 16, 1997·The Journal of Cell Biology·A SakakibaraS Tsukita
Nov 14, 1997·The American Journal of Physiology·S D SavkovicG Hecht
Feb 11, 1998·Clinical Microbiology Reviews·J P Nataro, J B Kaper
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·S WuC L Sears
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·K MoritaS Tsukita
Sep 14, 1999·The American Journal of Physiology·G Hecht, A Koutsouris
Apr 4, 2000·The Journal of Cell Biology·S Tsukita, M Furuse
Mar 10, 2001·The Journal of Clinical Investigation·B P McNamaraG Hecht
Apr 3, 2001·Nature Reviews. Molecular Cell Biology·S TsukitaM Itoh
Jan 31, 2002·Histochemistry and Cell Biology·Junichi KobayashiYosaburo Shibata

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Citations

Oct 12, 2012·Cancer Chemotherapy and Pharmacology·Hannah R WardillRachel J Gibson
Dec 21, 2010·Inflammation·Qiang ZhangJieshou Li
Feb 8, 2011·Antioxidants & Redox Signaling·Lena J JohnJörg-Dieter Schulzke
Feb 8, 2006·Current Opinion in Gastroenterology·Mike G LaukoetterAsma Nusrat
Jun 10, 2009·Infection and Immunity·Daniel MüllerM Alexander Schmidt
Mar 22, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·V K ViswanathanGail Hecht
Mar 2, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Michel FaustherMaryse Picher
Jul 9, 2008·World Journal of Gastroenterology : WJG·Inaya-Abdallah Hajj HusseinAbdo Jurjus
Jan 1, 2008·Interdisciplinary Perspectives on Infectious Diseases·Robert A Britton, James Versalovic
Oct 13, 2010·Annual Review of Physiology·Le ShenJerrold R Turner
Aug 26, 2014·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Sanjeev GumberFrancois Villinger
Feb 18, 2011·Canadian Journal of Physiology and Pharmacology·Andrew N FlynnAndre G Buret
Jul 12, 2012·Cellular and Molecular Life Sciences : CMLS·Takuya Suzuki
Mar 15, 2016·Frontiers in Microbiology·Bruno M Di Genova, Renata R Tonelli
Dec 22, 2015·Digestive Diseases and Sciences·Farokh R DemehriDaniel H Teitelbaum
Jun 30, 2009·The Journal of Allergy and Clinical Immunology·Katherine R Groschwitz, Simon P Hogan
Oct 5, 2007·The Journal of Investigative Dermatology·Ulrich OhnemusJohanna M Brandner
Sep 30, 2008·Trends in Microbiology·Julian A Guttman, B Brett Finlay
Dec 19, 2014·Scientific Reports·Sonja BlascheManfred Koegl
Jun 23, 2009·Annals of the New York Academy of Sciences·Andrew W WeflenGail A Hecht
Feb 25, 2009·The Journal of Pathology·Qiurong LiJieshou Li
Mar 22, 2006·Cellular Microbiology·Julian A GuttmanB Brett Finlay
Apr 19, 2007·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Julian A GuttmanB Brett Finlay
Jun 27, 2012·Annals of the New York Academy of Sciences·Lila G Glotfelty, Gail A Hecht
Apr 15, 2011·Molecular Microbiology·Alexander R C WongElizabeth L Hartland
Dec 9, 2008·Biochimica Et Biophysica Acta·Julian A Guttman, B Brett Finlay
Aug 17, 2005·Current Opinion in Cell Biology·Sandra SousaPascale Cossart
Jun 14, 2005·Comptes rendus biologies·Akio AbeAsaomi Kuwae
Oct 5, 2007·Biochimica Et Biophysica Acta·Dan Yu, Jerrold R Turner

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