Enterotoxin B synthesis by replicating and nonreplicating cells of Staphylococcus aureus.

Journal of Bacteriology
Z Markus, G J Silverman

Abstract

Although 95% of the enterotoxin B produced by Staphylococcus aureus appears during the latter part of the exponential phase of growth, growth per se is not necessary for toxin synthesis. A procedure is described whereby a concentrated suspension (at least 6 x 10(10) cells per ml) of a 16-hr culture of S. aureus was found to be capable of producing toxin, without replication, when air and glucose were present. This technique allows the growth requirement to be separated from toxin formation. Although higher (100 mug/ml) concentrations of toxin appeared in the medium when nitrogen was present, lower levels (30 mug/ml) were produced in the absence of N-Z-amine A. Toxin production proceeded without any net increase in deoxyribonucleic acid, ribonucleic acid, or protein. Chloramphenicol did not inhibit toxin formation in a nitrogen-free medium. The optimal pH for toxin production in a nitrogen-free medium was 8.0 to 8.5; for synthesis in a medium where nitrogen was available, the optimal pH was 7.0 to 7.5. Increasing the rate of aeration increased toxin release during growth, but decreased the amount of toxin subsequently produced when the bacteria were resuspended. These results suggest the presence of a precursor pool in the cells...Continue Reading

References

Mar 1, 1966·Applied Microbiology·F J WeiretherR E Lincoln
Dec 1, 1967·Japanese Journal of Microbiology·M S BergdollK F Weiss
Oct 1, 1968·Journal of Bacteriology·Z Markus, G J Silverman
Jul 1, 1966·Journal of Bacteriology·M E Friedman
Jul 1, 1966·Journal of Bacteriology·A J Rosenwald, R E Lincoln
Nov 1, 1966·Applied Microbiology·E KatoM S Bergdoll
Nov 1, 1949·The Journal of Experimental Medicine·A W BERNHEIMER

❮ Previous
Next ❯

Citations

Dec 1, 1972·The Journal of Hygiene·R J GilbertM Simkovicová
Apr 1, 1973·Applied Microbiology·S A Morse, R A Mah
Sep 1, 1970·Applied Microbiology·J A Troller, M A Bozeman
Sep 1, 1986·Applied and Environmental Microbiology·S B GoldnerL S Post
May 1, 1971·Applied Microbiology·C GenigeorgisW W Sadler
Oct 1, 1972·Applied Microbiology·G G DietrichG J Silverman
Jun 1, 1973·Infection and Immunity·S A Morse, J N Baldwin
Aug 1, 1971·Applied Microbiology·S A Morse, J N Baldwin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.