Enterotoxin-Encoding Genes in Staphylococcus spp. from Food Handlers in a University Restaurant

Foodborne Pathogens and Disease
Sabina Dos Santos Paulino da SilvaMaria Celeste Nunes de Melo

Abstract

Food handlers carrying enterotoxin-producing Staphylococcus are a potential source of food poisoning. The aim of this study was to analyze genes encoding enterotoxins in coagulase-positive Staphylococcus (CoPS) and coagulase-negative Staphylococcus (CoNS) isolated from the anterior nostrils and hands of food handlers at a university restaurant in the city of Natal, Northeast Brazil. Thirty food handlers were screened for the study. The isolates were subjected to Gram staining, a bacitracin sensitivity test, mannitol fermentation, and catalase and coagulase tests. CoNS and CoPS strains were subsequently identified by a Vitek 2 System (BioMerieux, France) and various biochemical tests. Polymerase chain reaction was used to detect genes for enterotoxins A, B, C, D, E, G, H, and I (sea, seb, sec, sed, see, seg, seh, and sei) and a disc-diffusion method was used to determine susceptibility to several classes of antimicrobials. All food handlers presented staphylococci on their hands and/or noses. The study found 58 Staphylococcus spp., of which 20.7% were CoPS and 79.3% were CoNS. S. epidermidis was the most prevalent species. Twenty-nine staphylococci (50%) were positive for one or more enterotoxin genes, and the most prevalent gen...Continue Reading

References

Jun 1, 1993·Epidemiology and Infection·A A WienekeR J Gilbert
Jul 1, 1996·International Journal of Food Microbiology·C Vernozy-RozandJ Fleurette
Mar 8, 2005·Infection·J A J W Kluytmans, H F L Wertheim
Jan 22, 2008·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Jamaira Fereira VerasMarti Jett
Oct 14, 2009·Journal of Public Health Management and Practice : JPHMP·Zeynep SimsekGulcan Gürses
Feb 28, 2013·Foodborne Pathogens and Disease·Daniele G LyraCelso J B Oliveira

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Citations

Oct 26, 2018·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Saeed FooladvandEhsanollah Ghaznavi-Rad

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