Abstract
Enterovirus 71 (EV71) is a pathogenic microorganism that causes hand, foot and mouth disease. However, the epigenetic mechanisms behind how EV71 regulates host cell proliferation and apoptosis are unclear. In the present study, the ability of EV71 to induce apoptosis was analyzed in the SH-SY5Y human neuroblastoma cell line and the effect of this virus on the mRNA expression levels of various apoptotic markers, miRNA let-7b and cyclin D1 (CCND1), was also investigated. The results demonstrated that EV71 induced SH-SY5Y cell apoptosis. An MTT assay revealed a significant inhibitory effect of EV71 on cell proliferation between 12-72 h post injection, compared with the control group. Furthermore, quantitative polymerase chain reaction and western blot analyses demonstrated that expression level of the apoptosis inhibitor Bcl-2 was markedly reduced, but the expression levels of the apoptosis-promoting factors Bax, caspase-7, caspase‑3 and active caspase-3 were markedly higher in the SH-SY5Y cells 12-48 h after EV71 infection, compared with the non-infected cells. In addition, flow cytometric assays revealed that EV71 arrested the cell cycle of host SH-SY5Y cells. Northern blot analysis revealed a marked miRNA let-7b hybridization s...Continue Reading
References
Jan 28, 2004·Cell·David P Bartel
Jun 24, 2004·Nature Reviews. Genetics·Lin He, Gregory J Hannon
Jun 22, 2007·Cellular Microbiology·Tsan-Chi ChenJyh-Lyh Juang
Dec 18, 2007·Cell·Fengyan YuErwei Song
Apr 2, 2008·Cell Research·Julia SchultzManfred Kunz
May 6, 2008·European Journal of Pharmacology·Zhigang WangDaling Zhu
Jul 2, 2008·Diabetes·Abdelfattah El OuaamariEmmanuel van Obberghen
Jan 21, 2009·The EMBO Journal·Surabhi Dangi-GarimellaMarsha Rich Rosner
Jun 9, 2009·Brain Research·Ping LeiJianning Zhang
Nov 6, 2009·Journal of Virology·Jennifer L Umbach, Bryan R Cullen
Feb 25, 2010·Current Oncology·D BarhP Sindhurani
Jul 14, 2010·Journal of Biomedicine & Biotechnology·Lunbiao CuiHua Wang
Mar 31, 2011·Development·John S PoultonWu-Min Deng
Apr 26, 2011·Human Reproduction·Yimin DaiYali Hu
Jul 15, 2011·Nature·Andrew S YooGerald R Crabtree
Sep 16, 2011·Journal of Virology·Christopher M NarbusMatthew J Evans
Oct 18, 2011·Cell·Pavel SumazinAndrea Califano
Nov 17, 2011·PloS One·Lunbiao CuiHua Wang
Dec 28, 2011·Experimental Cell Research·Te LiuLihe Guo
Feb 16, 2012·Annals of Botany·Zhenhua FengJian Zhu
Apr 6, 2012·PloS One·Wei HuShengqi Wang
Apr 14, 2012·The FEBS Journal·Weiwei ChengHui Wang
Oct 9, 2012·Nucleic Acids Research·Laurent HouzetKuan-Teh Jeang
Nov 15, 2012·Protein & Cell·Yongkui LiYing Zhu
Nov 24, 2012·International Journal of Molecular Medicine·Te LiuChuan Chen
Jan 19, 2013·Stem Cells and Development·Te LiuLihe Guo
Mar 8, 2013·Journal of Virology·Zhenhua ZhengHanzhong Wang
Mar 26, 2013·FEBS Letters·Wenxing QinJiejun Wang
Apr 19, 2013·Intervirology·Bai-ping WenRu Sheng
Citations
Nov 28, 2015·Viruses·Hsing-I Huang, Shin-Ru Shih
Jan 12, 2016·Viruses·Bing-Ching HoSung-Liang Yu
Aug 15, 2018·Critical Reviews in Microbiology·Ilka EngelmannDidier Hober
Sep 29, 2017·Journal of Virology·Mackenzie M ShipleyMoriah L Szpara
Nov 17, 2016·Journal of Virology·Jing LiJuan Tan
May 8, 2019·Viral Immunology·Hong ZhouYanjun Li
Nov 17, 2016·Scientific Reports·Issac Horng Khit TooSylvie Alonso
Oct 3, 2019·International Journal of Molecular Sciences·Huanru WangZhiwei Wu
Jan 15, 2020·Journal of Neurovirology·Oratai SupasornJeeraphong Thanongsaksrikul
Sep 21, 2018·Frontiers in Cellular and Infection Microbiology·Qing ZhaoBo Wang
Sep 30, 2018·Scientific Reports·Claudia Sánchez-San MartínCharles Y Chiu
Nov 30, 2018·Viruses·Chiaho ShihAn-Ting Liou
Jun 26, 2020·Frontiers in Microbiology·Yalan LaiXiaoyue Chen
Oct 19, 2017·Experimental and Therapeutic Medicine·Zengjun JinWeihao Li
Aug 4, 2016·Molecular Medicine Reports·Te LiuJin Zheng
Dec 29, 2019·Scientific Reports·Heloísa Antoniella Braz-De-MeloKelly Grace Magalhães
Aug 28, 2020·Archives of Virology·Meijuan ZhangShengfeng Qiu
May 22, 2020·Virulence·Lei YouJianguo Wu
Nov 24, 2016·Archives of Virology·Thinesshwary YogarajahKum Thong Wong
Aug 16, 2015·Brain Pathology·Kien Chai Ong, Kum Thong Wong
Mar 4, 2020·Scientific Reports·Siti Naqiah AmrunLisa F P Ng
Jan 23, 2021·PloS One·Menghuai SunZheng Xing
Apr 4, 2021·International Journal of Molecular Sciences·Peiyu ZhuYuefei Jin
Apr 27, 2021·Molecular Therapy. Nucleic Acids·Javid Sadri NahandHamed Mirzaei
Sep 19, 2021·Archives of Virology·Min GuoBing Zhu