Enterovirus 71 induces apoptosis of SH‑SY5Y human neuroblastoma cells through stimulation of endogenous microRNA let-7b expression

Molecular Medicine Reports
Xiling DuTe Liu

Abstract

Enterovirus 71 (EV71) is a pathogenic microorganism that causes hand, foot and mouth disease. However, the epigenetic mechanisms behind how EV71 regulates host cell proliferation and apoptosis are unclear. In the present study, the ability of EV71 to induce apoptosis was analyzed in the SH-SY5Y human neuroblastoma cell line and the effect of this virus on the mRNA expression levels of various apoptotic markers, miRNA let-7b and cyclin D1 (CCND1), was also investigated. The results demonstrated that EV71 induced SH-SY5Y cell apoptosis. An MTT assay revealed a significant inhibitory effect of EV71 on cell proliferation between 12-72 h post injection, compared with the control group. Furthermore, quantitative polymerase chain reaction and western blot analyses demonstrated that expression level of the apoptosis inhibitor Bcl-2 was markedly reduced, but the expression levels of the apoptosis-promoting factors Bax, caspase-7, caspase‑3 and active caspase-3 were markedly higher in the SH-SY5Y cells 12-48 h after EV71 infection, compared with the non-infected cells. In addition, flow cytometric assays revealed that EV71 arrested the cell cycle of host SH-SY5Y cells. Northern blot analysis revealed a marked miRNA let-7b hybridization s...Continue Reading

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Citations

Nov 28, 2015·Viruses·Hsing-I Huang, Shin-Ru Shih
Jan 12, 2016·Viruses·Bing-Ching HoSung-Liang Yu
Aug 15, 2018·Critical Reviews in Microbiology·Ilka EngelmannDidier Hober
Sep 29, 2017·Journal of Virology·Mackenzie M ShipleyMoriah L Szpara
Nov 17, 2016·Scientific Reports·Issac Horng Khit TooSylvie Alonso
Oct 3, 2019·International Journal of Molecular Sciences·Huanru WangZhiwei Wu
Sep 21, 2018·Frontiers in Cellular and Infection Microbiology·Qing ZhaoBo Wang
Sep 30, 2018·Scientific Reports·Claudia Sánchez-San MartínCharles Y Chiu
Jun 26, 2020·Frontiers in Microbiology·Yalan LaiXiaoyue Chen
Oct 19, 2017·Experimental and Therapeutic Medicine·Zengjun JinWeihao Li
Apr 4, 2021·International Journal of Molecular Sciences·Peiyu ZhuYuefei Jin
Apr 27, 2021·Molecular Therapy. Nucleic Acids·Javid Sadri NahandHamed Mirzaei

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