Enthalpic barriers to the hydrophobic binding of oligosaccharides to phage P22 tailspike protein.

Biochemistry
Ulrich BaxaR Seckler

Abstract

The structural thermodynamics of the recognition of complex carbohydrates by proteins are not well understood. The recognition of O-antigen polysaccharide by phage P22 tailspike protein is a highly suitable model for advancing knowledge in this field. The binding to octa- and dodecasaccharides derived from Salmonella enteritidis O-antigen was studied by isothermal titration calorimetry and stopped-flow spectrofluorimetry. At room temperature, the binding reaction is enthalpically driven with an unfavorable change in entropy. A large change of -1.8 +/- 0.2 kJ mol(-1) K(-1) in heat capacity suggests that the hydrophobic effect and water reorganization contribute substantially to complex formation. As expected from the large heat-capacity change, we found enthalpy-entropy compensation. The calorimetrically measured binding enthalpies were identical within error to van't Hoff enthalpies determined from fluorescence titrations. Binding kinetics were determined at temperatures ranging from 10 to 30 degrees C. The second-order association rate constant varied from 1 x 10(5) M(-1) s(-1) for dodecasaccharide at 10 degrees C to 7 x 10(5) M(-1) s(-1) for octasaccharide at 30 degrees C. The first-order dissociation rate constants ranged fr...Continue Reading

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Citations

Jun 25, 2002·Journal of Molecular Biology·Alisdair B BorastonGideon J Davies
Sep 8, 2010·The Journal of Biological Chemistry·Dorothee AndresRobert Seckler
Jan 17, 2009·Macromolecular Bioscience·Stefanie BarbirzRobert Seckler
Apr 24, 2007·Biopolymers·Debabani Ganguly, Chaitali Mukhopadhyay
Apr 12, 2005·Archives of Biochemistry and Biophysics·Nabil Ali Mohammed Sultan, Musti J Swamy
Jan 22, 2005·Virology·Bärbel KaufmannRobert Seckler
Jun 25, 2004·The Biochemical Journal·Alisdair B BorastonGideon J Davies
Sep 25, 2010·Biochemical Society Transactions·Dorothee AndresStefanie Barbirz
May 21, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Hitesh HandaGuangzhao Mao
Jul 26, 2018·Journal of the American Chemical Society·Sonja KunstmannStefanie Barbirz

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