Entrapment of the Fastest Known Carbonic Anhydrase with Biomimetic Silica and Its Application for CO2 Sequestration.

Chia-Jung HsiehChi-Yang Yu


Capturing and storing CO2 is of prime importance. The rate of CO2 sequestration is often limited by the hydration of CO2, which can be greatly accelerated by using carbonic anhydrase (CA, EC as a catalyst. In order to improve the stability and reusability of CA, a silica-condensing peptide (R5) was fused with the fastest known CA from Sulfurihydrogenibium azorense (SazCA) to form R5-SazCA; the fusion protein successfully performed in vitro silicification. The entrapment efficiency reached 100% and the silicified form (R5-SazCA-SP) showed a high activity recovery of 91%. The residual activity of R5-SazCA-SP was two-fold higher than that of the free form when stored at 25 °C for 35 days; R5-SazCA-SP still retained 86% of its activity after 10 cycles of reuse. Comparing with an uncatalyzed reaction, the time required for the onset of CaCO3 formation was shortened by 43% and 33% with the addition of R5-SazCA and R5-SazCA-SP, respectively. R5-SazCA-SP shows great potential as a robust and efficient biocatalyst for CO2 sequestration because of its high activity, high stability, and reusability.


Sep 9, 2000·FEMS Microbiology Reviews·K S Smith, J G Ferry
Jan 6, 2004·Chemical Communications : Chem Comm·Marc R Knecht, David W Wright
Jan 13, 2004·Nature Biotechnology·Heather R LuckariftMorley O Stone
Oct 6, 2007·Biological Trace Element Research·Yanyou WuHuakun Huang
Dec 15, 2007·Biotechnology and Bioengineering·Liang-Jung Chien, Cheng-Kang Lee
Sep 2, 2008·Trends in Biotechnology·Lorena Betancor, Heather R Luckarift
Jul 24, 2010·Angewandte Chemie·Deanna M D'AlessandroJeffrey R Long
Jul 19, 2012·Journal of Enzyme Inhibition and Medicinal Chemistry·Clemente CapassoMosè Rossi
Dec 19, 2012·Chemical Communications : Chem Comm·Claire ForsythSiddharth V Patwardhan
Jul 18, 2015·International Journal of Molecular Sciences·Anna Di FioreKatia D'Ambrosio
Sep 28, 2018·World Journal of Microbiology & Biotechnology·Makoto Yoshimoto, Peter Walde
Apr 7, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Laura SeniorPaul Curnow

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