Entry from the cell surface of severe acute respiratory syndrome coronavirus with cleaved S protein as revealed by pseudotype virus bearing cleaved S protein.

Journal of Virology
Rie WatanabeFumihiro Taguchi

Abstract

Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is known to take an endosomal pathway for cell entry; however, it is thought to enter directly from the cell surface when a receptor-bound virion spike (S) protein is affected by trypsin, which induces cleavage of the S protein and activates its fusion potential. This suggests that SARS-CoV bearing a cleaved form of the S protein can enter cells directly from the cell surface without trypsin treatment. To explore this possibility, we introduced a furin-like cleavage sequence in the S protein at amino acids 798 to 801 and found that the mutated S protein was cleaved and induced cell fusion without trypsin treatment when expressed on the cell surface. Furthermore, a pseudotype virus bearing a cleaved S protein was revealed to infect cells in the presence of a lysosomotropic agent as well as a protease inhibitor, both of which are known to block SARS-CoV infection via an endosome, whereas the infection of pseudotypes with an uncleaved, wild-type S protein was blocked by these agents. A heptad repeat peptide, derived from a SARS-CoV S protein that is known to efficiently block infections from the cell surface, blocked the infection by a pseudotype with a cleaved S prot...Continue Reading

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Citations

Mar 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sandrine BelouzardGary R Whittaker
May 27, 2011·Journal of Virology·Kazuya ShiratoFumihiro Taguchi
Oct 31, 2008·Journal of Virology·Miyuki KawaseFumihiro Taguchi
Mar 12, 2010·Uirusu·Fumihiro Taguchi, Shutoku Matsuyama
Jul 21, 2012·Viruses·Sandrine BelouzardGary R Whittaker
Apr 25, 2014·Journal of Medical Virology·Kazuya ShiratoFumihiro Taguchi
Mar 7, 2009·The Journal of General Virology·Christel Schwegmann-WeßelsGeorg Herrler
May 17, 2012·Viruses·Taylor Heald-Sargent, Tom Gallagher
Jan 1, 2016·Toxicology and Environmental Health Sciences·Simranjeet Singh SekhonYang-Hoon Kim
Oct 17, 2020·Annals of Laboratory Medicine·Sarita ChoudharyPraveen Sharma
Feb 3, 2021·International Journal of Molecular Sciences·Nicholas A Wong, Milton H Saier
Aug 31, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Katya UzunovaToni Vekov
Apr 13, 2021·Virus Research·Thalia Rodriguez, Hana M Dobrovolny
Jun 22, 2021·Frontiers in Immunology·Jon Hazeldine, Janet M Lord
Jun 24, 2020·Drug Discovery Today·Vaibhav TiwariUmesh R Desai

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